Immunofluorescence analysis of Phospho-Tau pSer199 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Tau pSer199 Antibody (44734g) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog, Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 199. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Dendritic Spine Loss and Chronic White Matter Inflammation in a Mouse Model of Highly Repetitive Head Trauma.
44-734G was used in western blot to characterize chronic white matter inflammation and dendritic spine loss in a mouse model of highly repetitive head trauma
|Winston CN,Noël A,Neustadtl A,Parsadanian M,Barton DJ,Chellappa D,Wilkins TE,Alikhani AD,Zapple DN,Villapol S,Planel E,Burns MP||The American journal of pathology (186:552)||2016|
|Not Applicable||Not Cited||
Rapid alteration of protein phosphorylation during postmortem: implication in the study of protein phosphorylation.
44-734G was used in western blot to characterize postmortem behavior and the rapid alteration of protein phosphorylation
|Wang Y,Zhang Y,Hu W,Xie S,Gong CX,Iqbal K,Liu F||Scientific reports (5:null)||2015|
Neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau derived from Alzheimer's disease brain.
44-734G was used in western blot to research Alzheimer's disease brain and neuronal uptake and propagation of a rare phosphorylated high-molecular-weight tau
|Takeda S,Wegmann S,Cho H,DeVos SL,Commins C,Roe AD,Nicholls SB,Carlson GA,Pitstick R,Nobuhara CK,Costantino I,Frosch MP,Müller DJ,Irimia D,Hyman BT||Nature communications (6:null)||2015|
Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8).
44-734G was used in western blot to characterize cognitive and neuropathological Alzheimer's disease markers in a novel mouse model
|Porquet D,Andrés-Benito P,Griñán-Ferré C,Camins A,Ferrer I,Canudas AM,Del Valle J,Pallàs M||Age (Dordrecht, Netherlands) (37:null)||2015|
Cross talk between PI3K-AKT-GSK-3ß and PP2A pathways determines tau hyperphosphorylation.
44-734G was used in western blot to study the role of glycogen synthase kinase-3beta and protein phosphatase 2A in regulation of tau hyperphosphorylation
|Wang Y,Yang R,Gu J,Yin X,Jin N,Xie S,Wang Y,Chang H,Qian W,Shi J,Iqbal K,Gong CX,Cheng C,Liu F||Neurobiology of aging (36:188)||2015|
Early alterations in energy metabolism in the hippocampus of APPswe/PS1dE9 mouse model of Alzheimer's disease.
44-734G was used in western blot to investigate the abnormalites in hippocampal energy metabolism in the pathogenesis of Alzheimer disease.
|Pedrós I,Petrov D,Allgaier M,Sureda F,Barroso E,Beas-Zarate C,Auladell C,Pallàs M,Vázquez-Carrera M,Casadesús G,Folch J,Camins A||Biochimica et biophysica acta (1842:1556)||2014|
Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3¿/ß.
44-734G was used in western blot to study the lack of involvementof GSK3-alpha/beta in the elevated tau phosphorylation observed in Tau.P30L hypothermic mice
|Maurin H,Lechat B,Borghgraef P,Devijver H,Jaworski T,Van Leuven F||The European journal of neuroscience (40:2442)||2014|
Neurological characterization of mice deficient in GSK3¿ highlight pleiotropic physiological functions in cognition and pathological activity as Tau kinase.
44-734G was used in western blot to investigate the contribution of GSK3α to neurological diseases.
|Maurin H,Lechat B,Dewachter I,Ris L,Louis JV,Borghgraef P,Devijver H,Jaworski T,Van Leuven F||Molecular brain (6:null)||2013|
Age-dependent effects of A53T alpha-synuclein on behavior and dopaminergic function.
44-734G was used in western blot to use a mouse model to examine motor activity, anxiety-like, and depressive-like behaviors both before and after the onset of Parkinson's disease
|Oaks AW,Frankfurt M,Finkelstein DI,Sidhu A||PloS one (8:null)||2013|
Asp664 cleavage of amyloid precursor protein induces tau phosphorylation by decreasing protein phosphatase 2A activity.
44-734G was used in western blot to investigate the effect of caspase cleavage of APP on tau phosphorylation in relation to Aβ.
|Park SS,Jung HJ,Kim YJ,Park TK,Kim C,Choi H,Mook-Jung IH,Koo EH,Park SA||Journal of neurochemistry (123:856)||2012|
Methylthioninium chloride (methylene blue) induces autophagy and attenuates tauopathy in vitro and in vivo.
44-734G was used in western blot to report that autophagy is a mechanism by which methylene blue reduces tau levels.
|Congdon EE,Wu JW,Myeku N,Figueroa YH,Herman M,Marinec PS,Gestwicki JE,Dickey CA,Yu WH,Duff KE||Autophagy (8:609)||2012|
|The carboxy-terminal fragment of inhibitor-2 of protein phosphatase-2A induces Alzheimer disease pathology and cognitive impairment.||Wang X,Blanchard J,Kohlbrenner E,Clement N,Linden RM,Radu A,Grundke-Iqbal I,Iqbal K||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (24:4420)||2010|
|Developmental regulation of tau phosphorylation, tau kinases, and tau phosphatases.||Yu Y,Run X,Liang Z,Li Y,Liu F,Liu Y,Iqbal K,Grundke-Iqbal I,Gong CX||Journal of neurochemistry (108:1480)||2009|
|Human||Not Cited||Dephosphorylation of tau by protein phosphatase 5: impairment in Alzheimer's disease.||Liu F,Iqbal K,Grundke-Iqbal I,Rossie S,Gong CX||The Journal of biological chemistry (280:1790)||2005|
An experimental rat model of sporadic Alzheimer's disease and rescue of cognitive impairment with a neurotrophic peptide.
44-734G was used in immunohistochemistry - free floating and western blot to report that expression of the N-terminal I2NTF and C-terminal I2CTF halves of I2PP2A in rat brain reproduced key features of Alzheimer's disease
|Bolognin S,Blanchard J,Wang X,Basurto-Islas G,Tung YC,Kohlbrenner E,Grundke-Iqbal I,Iqbal K||Acta neuropathologica (123:133)||2012|
Co-localization of hyperphosphorylated tau and caspases in the brainstem of Alzheimer's disease patients.
44-734G was used in immunohistochemistry to compare the distribution and number of neurons with hyperphosphorylated tau in two age groups of Alzheimer's disease brainstems
|Wai MS,Liang Y,Shi C,Cho EY,Kung HF,Yew DT||Biogerontology (10:457)||2009|