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Up-regulation and Antibody-Peptide Competition. Human recombinant Tau stimulated with GSK-3β (1 µg per µg Tau) for 45 minutes was added to background extracts, resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and either left untreated (1-4) or treated with lambda phosphatase (5), then incubated with the Tau [pS422] antibody in a 3% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1,5), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP conjugate (Cat. no. ALI4404) and signals were detected using the Pierce SuperSignal™ method.The data show that only the phosphopeptide corresponding to Tau [pS422] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific. (Cat. No. 44764G)
|Tested species reactivity||Human|
|Published species reactivity||Tag, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Tau that contains serine 422. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||Not Cited||Targeting phospho-Ser422 by active Tau Immunotherapy in the THYTau22 mouse model: a suitable therapeutic approach.||Troquier L,Caillierez R,Burnouf S,Fernandez-Gomez FJ,Grosjean ME,Zommer N,Sergeant N,Schraen-Maschke S,Blum D,Buee L||Current Alzheimer research (9:397)||2012|
Methylthioninium chloride (methylene blue) induces autophagy and attenuates tauopathy in vitro and in vivo.
44-764G was used in western blot to report that autophagy is a mechanism by which methylene blue reduces tau levels.
|Congdon EE,Wu JW,Myeku N,Figueroa YH,Herman M,Marinec PS,Gestwicki JE,Dickey CA,Yu WH,Duff KE||Autophagy (8:609)||2012|
|Human||Not Cited||Nature of "Tau" immunoreactivity in normal myonuclei and inclusion body myositis.||Salajegheh M,Pinkus JL,Nazareno R,Amato AA,Parker KC,Greenberg SA||Muscle & nerve (40:520)||2009|
|Mouse||Not Cited||Increased tau phosphorylation on mitogen-activated protein kinase consensus sites and cognitive decline in transgenic models for Alzheimer's disease and FTDP-17: evidence for distinct molecular processes underlying tau abnormalities.||Lambourne SL,Sellers LA,Bush TG,Choudhury SK,Emson PC,Suh YH,Wilkinson LS||Molecular and cellular biology (25:278)||2005|
|Pseudophosphorylation of tau protein alters its ability for self-aggregation.||Haase C,Stieler JT,Arendt T,Holzer M||Journal of neurochemistry (88:1509)||2004|
G protein beta1/gamma2 subunit-interacting factor 1; MAPT; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; MTBT1; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; protein phosphatase 1, regulatory subunit 103
DDPAC; FTDP-17; MAPT; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU