Western blot analysis of Phospho-p70 S6 Kinase pThr389 in extracts from NIH/3T3 cells, serum-starved or serum-treated (20%), using Phospho-p70 S6 Kinase pThr389 monoclonal antibody (Product # MA5-15202) (upper) or a p70 S6 Kinase polyclonal antibody (lower).
|Tested species reactivity||Fruit fly, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pThr389 of human p70 S6 kinase|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.