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Invitrogen
Profilin 1 Monoclonal Antibody (OTI1D5)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 17
Profilin 1 Antibody (MA5-25113) in ICC/IF
Immunofluorescence of Profilin 1 (PFN1) was performed using HAP1 WT and PFN1 KO cells that were labeled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT an... View More
Product Details
MA5-25113
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI1D5
Immunogen
Full length human recombinant protein of PFN1 produced in HEK293T cell
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2725111
Target Information
Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Epididymis tissue protein Li 184a; Profilin I; Profilin-1
Gene Aliases: ALS18; PFN1
UniProt ID: (Human) P07737, (Rat) P62963
Entrez Gene ID: (Dog) 607397, (Human) 5216, (Rat) 64303
adenyl-nucleotide exchange factor activity
actin binding
actin monomer binding
receptor binding
protein binding
phosphatidylinositol-4,5-bisphosphate binding
Rho GTPase binding
poly(A) RNA binding
proline-rich region binding
cadherin binding involved in cell-cell adhesion
actin or actin-binding cytoskeletal protein
cytoskeletal protein
non-motor actin binding protein
neural tube closure
positive regulation of epithelial cell migration
actin cytoskeleton organization
negative regulation of actin filament polymerization
positive regulation of actin filament polymerization
negative regulation of actin filament bundle assembly
positive regulation of actin filament bundle assembly
positive regulation of ATPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
protein stabilization
positive regulation of DNA metabolic process
positive regulation of stress fiber assembly
negative regulation of stress fiber assembly
Wnt signaling pathway, planar cell polarity pathway
cellular response to growth factor stimulus
cell-cell adhesion
positive regulation of ruffle assembly
regulation of transcription from RNA polymerase II promoter
actin polymerization or depolymerization
response to organic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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