Invitrogen

RAB17 Monoclonal Antibody (OTI5E2)

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RAB17 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of RAB17 in COS7 cells. Cells were transfected with a plasmid overexpressing RAB17 and probed with a RAB17 monoclonal antibody (Product # MA5-25117).

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a RAB17 monoclonal antibody (Product # MA5-25117) at a dilution of 1:50.

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a RAB17 monoclonal antibody (Product # MA5-25117) at a dilution of 1:50.

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a RAB17 monoclonal antibody (Product # MA5-25117) at a dilution of 1:50.

RAB17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a RAB17 monoclonal antibody (Product # MA5-25117) at a dilution of 1:50.

RAB17 Antibody in Western Blot (WB) — Western blot analysis of RAB17 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with RAB17 (Product # MA5-25117) monoclonal antibody.

Product Details

MA5-25117

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI5E2
Immunogen	Full length human recombinant protein of RAB17 produced in HEK293T cell View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.63 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2725168

Target Information

The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ras-related protein Rab-17

Gene Aliases: RAB17

UniProt ID: (Human) Q9H0T7

Entrez Gene ID: (Human) 64284

immunoglobulin transcytosis in epithelial cells mediated by polymeric immunoglobulin receptor small GTPase mediated signal transduction protein transport regulation of endocytosis establishment of melanosome localization melanosome transport endocytic recycling cilium assembly transcytosis filopodium assembly regulation of dendrite development regulation of filopodium assembly regulation of synapse assembly

Process(es)

GTPase activity protein binding GTP binding GDP binding