Invitrogen

RAB3A Monoclonal Antibody (OTI5C12)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

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RAB3A Antibody in Western Blot (WB) — Western blot was performed using Anti-RAB3A Mouse Monoclonal Antibody (OTI5C12) (Product # MA5-27178) and a 25 kDa band corresponding to RAB3A was observed across tissues tested except Rat kidney and Rat liver. Whole cell extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Rat kidney (Lane 3) and Rat liver (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

RAB3A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human prostate tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a RAB3A monoclonal antibody (Product # MA5-27178) at a dilution of 1:150.

RAB3A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human pancreas tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a RAB3A monoclonal antibody (Product # MA5-27178) at a dilution of 1:150.

RAB3A Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of RAB3A was observed in Mouse brain, Rat brain in comparison to Rat kidney and Rat liver using Anti-RAB3A Mouse Monoclonal Antibody (OTI5C12) (Product # MA5-27178) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000-1:2000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:150

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI5C12

Immunogen

Full length human recombinant protein of RAB3A produced in E.coli

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2725179

Target Information

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D. These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters. Specifically, Rab 3s are thought to play a key role in presynaptic vesicle trafficking and priming.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C18A3.6; RAS-associated protein RAB3A; Ras-related protein Rab-3A; Ras-related small GTP binding protein 3A

Gene Aliases: RAB3; RAB3A

UniProt ID: (Human) P20336, (Rat) P63012, (Mouse) P63011

Entrez Gene ID: (Human) 5864, (Rat) 25531, (Mouse) 19339

respiratory system process intracellular protein transport vesicle docking involved in exocytosis mitochondrion organization neuromuscular synaptic transmission axonogenesis metabolic process protein secretion post-embryonic development synaptic vesicle exocytosis synaptic vesicle maturation regulation of exocytosis lung development regulation of synaptic vesicle fusion to presynaptic membrane Rab protein signal transduction positive regulation of ATPase activity synaptic vesicle recycling regulation of short-term neuronal synaptic plasticity maintenance of presynaptic active zone structure sensory perception of touch response to electrical stimulus protein localization to plasma membrane positive regulation of regulated secretory pathway transport exocytosis small GTPase mediated signal transduction protein transport synaptic vesicle transport evoked neurotransmitter secretion regulation of long-term synaptic potentiation neurotransmitter secretion glutamate secretion constitutive secretory pathway positive regulation of exocytosis

Process(es)

ATPase activator activity GTPase activity protein binding GTP binding protein C-terminus binding myosin V binding GTPase binding ATPase binding GDP binding nucleotide binding