Invitrogen

RAD50 Monoclonal Antibody (13B3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

1 Reference

View all (19) RAD50 antibodies

Datasheet

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Datasheet

Questions & Answers

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RAD50 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of RAD50 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

RAD50 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of RAD50 was performed in paraffin-embedded human lung cancer tissue using RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) at a dilution of 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

RAD50 Antibody in Western Blot (WB) — Western Blot analysis of RAD50 was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) at a dilution of 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

RAD50 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. 293T cells were transfected with RAD50 siRNA and decrease in signal intensity was observed in western blot application using RAD50 Antibody (Product # MA1-23269). {KD}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (IHC)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay dependent

In vitro Assay (IV)

Assay Dependent

Western Blot (WB)

1:500-1:3,000

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

13B3

Immunogen

Amino acids 1-425 of Rad50 expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_560822

Product Specific Information

Recommended positive controls: HeLa, HeLa nuclear extract.

Target Information

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA repair protein RAD50; hRAD50; hsRAD50; mRad50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3

Gene Aliases: hRad50; Mrell; NBSLD; RAD50; RAD502; Rad50l

UniProt ID: (Human) Q92878, (Rat) Q9JIL8

Entrez Gene ID: (Human) 10111, (Mouse) 19360, (Rat) 64012

Function(s)