Invitrogen

RAD50 Monoclonal Antibody (13B3)

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This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (15) RAD50 antibodies

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RAD50 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of RAD50 in HeLa cells using a RAD50 monoclonal antibody (Product # MA1-23269) at a 1:100 dilution.

RAD50 Antibody in Western Blot (WB) — Western blot analysis of Rad50 in HeLa whole cell and nuclear extracts (30 µg). Samples were separated by 5% SDS-PAGE and the membrane was probed with Rad50, N-term Monoclonal antibody (Product # MA1-23269) at a dilution of 1:500.

RAD50 Antibody in Western Blot (WB) — Western blot analysis of RAD50 using 30 µg HeLa whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a RAD50 monoclonal antibody (Product # MA1-23269) at a dilution of 1:1000.

RAD50 Antibody in Western Blot (WB) — Western blot analysis of RAD50 using a RAD50 monoclonal antibody (Product # MA1-23269).

RAD50 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. 293T cells were transfected with RAD50 siRNA and decrease in signal intensity was observed in western blot application using RAD50 Antibody (Product # MA1-23269). {KD}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

Assay-dependent

Immunofluorescence (IF)

1:5000

Immunohistochemistry (IHC)

Assay-dependent

Immunoprecipitation (IP)

Assay dependent

Western Blot (WB)

1:500-3000

Published Applications

Miscellaneous PubMed (MISC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

13B3

Immunogen

Amino acids 1-425 of Rad50 expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_560822

Product Specific Information

MA1-23269 detects Rad50 in human samples.

MA1-23269 has been successfully used in immunofluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures.

MA1-23269 immunogen is residues 1-425 of Rad50 expressed in E. coli..

Target Information

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA repair protein RAD50; hRAD50; mRad50; NBSLD; RAD50 homolog, double strand break repair protein; RAD50-2

Gene Aliases: hRad50; Mrell; NBSLD; RAD50; RAD502; Rad50l

UniProt ID: (Human) Q92878, (Rat) Q9JIL8

Entrez Gene ID: (Human) 10111, (Mouse) 19360, (Rat) 64012

regulation of mitotic recombination telomere maintenance DNA repair double-strand break repair DNA recombination cellular response to DNA damage stimulus telomere maintenance via telomerase cell cycle regulation of mitotic cell cycle positive regulation of protein autophosphorylation DNA duplex unwinding positive regulation of kinase activity negative regulation of viral entry into host cell chromosome organization protein heterooligomerization meiotic cell cycle chromosome organization involved in meiotic cell cycle nucleic acid phosphodiester bond hydrolysis DNA catabolic process, endonucleolytic reciprocal meiotic recombination telomere maintenance via recombination double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement DNA replication double-strand break repair via nonhomologous end joining viral process telomeric 3' overhang formation positive regulation of telomere maintenance nucleotide phosphorylation regulation of signal transduction by p53 class mediator negative regulation of telomere capping

Process(es)

single-stranded DNA endodeoxyribonuclease activity DNA binding double-stranded telomeric DNA binding ATP-dependent DNA helicase activity adenylate kinase activity protein binding ATP binding 3'-5' exonuclease activity protein binding, bridging single-stranded telomeric DNA binding metal ion binding G-quadruplex DNA binding nucleotide binding double-stranded DNA binding hydrolase activity ATPase activity