Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | - |
Immunoprecipitation (IP) |
Assay-dependent | View 1 publication 1 publication |
In vitro Assay (IV) |
Assay-dependent | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate, Rat |
Published species |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
13B3 |
Immunogen |
Amino acids 1-425 of Rad50 expressed in E. coli. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
2.24 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_560822 |
Recommended positive controls: HeLa, HeLa nuclear extract.
Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA repair protein RAD50; hRAD50; hsRAD50; mRad50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3
Gene Aliases: hRad50; Mrell; NBSLD; RAD50; RAD502; Rad50l
UniProt ID: (Human) Q92878, (Rat) Q9JIL8
Entrez Gene ID: (Human) 10111, (Mouse) 19360, (Rat) 64012
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