Invitrogen

RAD50 Monoclonal Antibody (13B3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (22) RAD50 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

RAD50 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of RAD50 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

RAD50 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of RAD50 was performed in paraffin-embedded human lung cancer tissue using RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) at a dilution of 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

RAD50 Antibody in Western Blot (WB) — Western Blot using RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269). HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

RAD50 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of target protein. HeLa cells were transfected with RAD50 shRNA and decrease in signal intensity was observed in Western blotting using Anti-RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269). {KD}

Product Details

MA1-23269

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication
In vitro Assay (IV)	Assay-dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	13B3
Immunogen	Amino acids 1-425 of Rad50 expressed in E. coli. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.24 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_560822

Product Specific Information

Recommended positive controls: HeLa, HeLa nuclear extract.

Target Information

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of tumor suppressor inositol polyphosphate 4-phosphatase type B impairs DNA double-strand break repair by destabilization of DNA tethering protein Rad50.

Cell death & disease

Sun Y,Ning X,Fan J,Hu J,Jiang Y,Hu Z,Paulo JA,Liu J,Qiu X,Xu H,Fu S,Gygi SP,Zhang J,Zhou C

MA1-23269 was used in Immunoprecipitation to investigate the role of inositol polyphosphate 4-phosphatase type B (INPP4B) as a tumour suppressor and its role in DNA break repair by stabilising Rad50.

Mon Apr 27 00:00:00 EDT 2020

Loss of tumor suppressor inositol polyphosphate 4-phosphatase type B impairs DNA double-strand break repair by destabilization of DNA tethering protein Rad50.

Cell death & disease

Sun Y,Ning X,Fan J,Hu J,Jiang Y,Hu Z,Paulo JA,Liu J,Qiu X,Xu H,Fu S,Gygi SP,Zhang J,Zhou C

MA1-23269 was used in Immunoprecipitation to investigate the role of inositol polyphosphate 4-phosphatase type B (INPP4B) as a tumour suppressor and its role in DNA break repair by stabilising Rad50.

Mon Apr 27 00:00:00 EDT 2020

Positional stability of single double-strand breaks in mammalian cells.

Nature cell biology

Soutoglou E,Dorn JF,Sengupta K,Jasin M,Nussenzweig A,Ried T,Danuser G,Misteli T

MA1-23269 was used in immunocytochemistry and western blot to study the mechanism for chromosome translocation in living mammalian cells

Fri Jun 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: DNA repair protein RAD50; hRAD50; hsRAD50; mRad50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3

Gene Aliases: hRad50; Mrell; NBSLD; RAD50; RAD502; Rad50l

UniProt ID: (Human) Q92878, (Rat) Q9JIL8

Entrez Gene ID: (Human) 10111, (Mouse) 19360, (Rat) 64012

Function(s)