Invitrogen

RAD50 Monoclonal Antibody (13B3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

2 References

View all (21) RAD50 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

RAD50 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of RAD50 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

RAD50 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of RAD50 was performed in paraffin-embedded human lung cancer tissue using RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) at a dilution of 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

RAD50 Antibody in Western Blot (WB) — Western Blot analysis of RAD50 was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) at a dilution of 1:1000. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

RAD50 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of target protein. HeLa cells were transfected with RAD50 shRNA and decrease in signal intensity was observed in Western blotting using Anti-RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269). {KD}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

13B3

Immunogen

Amino acids 1-425 of Rad50 expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.24 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_560822

Product Specific Information

Recommended positive controls: HeLa, HeLa nuclear extract.

Target Information

Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA repair protein RAD50; hRAD50; hsRAD50; mRad50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3

Gene Aliases: hRad50; Mrell; NBSLD; RAD50; RAD502; Rad50l

UniProt ID: (Human) Q92878, (Rat) Q9JIL8

Entrez Gene ID: (Human) 10111, (Mouse) 19360, (Rat) 64012

Function(s)