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Recoverin Antibody (MA1-932) in ICC/IF
Immunofluorescent analysis of Recoverin (green) showing staining in the cytoplasm of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Recoverin monoclonal antibody (Product # MA1-932) in 3% BSA-PBS at ... View More
Product Details
MA1-932
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Sheep,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
6a55 cd6
Immunogen
Proprietary Immunogen.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2178006
Product Specific Information
MA1-932 will detect Recoverin from human, rat, bovine, and ovine samples.
MA1-932 has been successfully used in Western blot, ICC/IF and IHC (P) procedures. By Western blot, this antibody will detect a ~23 kDa band representing Recoverin from rat brain, as well as in bovine and ovine retina. This antibody is not recommended for rat brain tissue in IHC applications.
The MA1-932 immunogen is considered commercially sensitive.
Target Information
Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cancer associated retinopathy antigen; Cancer-associated retinopathy protein; p26; Protein CAR; Recoverin
Gene Aliases: RCV1; RCVRN
UniProt ID: (Bovine) P21457, (Human) P35243
Entrez Gene ID: (Sheep) 101111206, (Bovine) 281447, (Human) 5957, (Rat) 140936
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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