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Invitrogen
Ryanodine Receptor Monoclonal Antibody (C3-33)
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Product Details
MA3-916
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Amphibian,
Dog,
Chicken,
Fish,
Guinea pig,
Human,
Mouse,
Rabbit,
Rat
Published species
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Sheep,
Zebrafish
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C3-33
Immunogen
Canine cardiac ryanodine receptor (RyR2)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-916 detects ryanodine receptor 2 (RyR2) and shows cross-reactivity with RyR1 in amphibian, canine, chicken, fish, guinea pig and rat tissues.
MA3-916 has been successfully used in Western blot, immunohistochemical, immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in rat cardiac tissue with MA3-916 results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.
Target Information
The ryanodine receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ca2+ release channel; calcium release channel; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; cardiac muscle-type ryanodine receptor; cardiac ryanodine receptor; cardiac ryanodine receptor 2; cardiac-type ryanodine receptor; central core disease of muscle; islet-type ryanodine receptor; kidney-type ryanodine receptor; protein phosphatase 1, regulatory subunit 137; Ryanodine receptor 1; ryanodine receptor 1 (skeletal); ryanodine receptor 1, skeletal muscle; ryanodine receptor 1-like, skeletal muscle; Ryanodine receptor 2; ryanodine receptor 2 (cardiac); ryanodine receptor type 2; ryanodine receptor type II; RYR-1; RYR-2; sarcoplasmic reticulum calcium release channel; Skeletal muscle calcium release channel; Skeletal muscle ryanodine receptor; Skeletal muscle-type ryanodine receptor; Type 1 ryanodine receptor; type 1-like ryanodine receptor; Type 2 ryanodine receptor; unnamed protein product
Gene Aliases: 9330127I20Rik; AI528790; ARVC2; ARVD2; CCO; MHS; MHS1; PPP1R137; RYDR; RYR; RYR-1; RYR-2; RYR1; Ryr1l; RYR2; SKRR; VTSIP
UniProt ID: (Rabbit) P11716, (Human) P21817, (Human) Q92736, (Mouse) E9PZQ0, (Rat) B0LPN4, (Mouse) E9Q401
protease binding
ryanodine-sensitive calcium-release channel activity
voltage-gated calcium channel activity
calcium channel activity
calcium ion binding
protein binding
calmodulin binding
calcium-release channel activity
enzyme binding
protein kinase A catalytic subunit binding
protein kinase A regulatory subunit binding
identical protein binding
protein self-association
suramin binding
ion channel binding
calcium-induced calcium release activity
ion channel activity
protein kinase binding
scaffold protein binding
organic cyclic compound binding
ligand-gated ion channel
scaffold/adaptor protein
response to hypoxia
outflow tract morphogenesis
calcium ion transport
muscle contraction
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to caffeine
ion transmembrane transport
skin development
ossification involved in bone maturation
skeletal muscle fiber development
release of sequestered calcium ion into cytosol
regulation of cytosolic calcium ion concentration
cellular response to caffeine
regulation of cardiac conduction
regulation of heart rate
embryonic heart tube morphogenesis
left ventricular cardiac muscle tissue morphogenesis
cardiac muscle hypertrophy
detection of calcium ion
cellular calcium ion homeostasis
positive regulation of heart rate
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
regulation of cardiac muscle contraction by calcium ion signaling
response to muscle activity
calcium-mediated signaling
BMP signaling pathway
calcium-mediated signaling using intracellular calcium source
response to muscle stretch
positive regulation of sequestering of calcium ion
response to redox state
regulation of cardiac muscle contraction
cardiac muscle contraction
canonical Wnt signaling pathway
calcium ion transport into cytosol
sarcoplasmic reticulum calcium ion transport
cellular response to epinephrine stimulus
establishment of protein localization to endoplasmic reticulum
ventricular cardiac muscle cell action potential
Purkinje myocyte to ventricular cardiac muscle cell signaling
cell communication by electrical coupling involved in cardiac conduction
type B pancreatic cell apoptotic process
positive regulation of the force of heart contraction
regulation of AV node cell action potential
regulation of SA node cell action potential
regulation of atrial cardiac muscle cell action potential
regulation of ventricular cardiac muscle cell action potential
positive regulation of calcium-transporting ATPase activity
transport
ion transport
regulation of muscle contraction
multicellular organism development
regulation of ion transmembrane transport
transmembrane transport
calcium ion transmembrane transport
response to nutrient
response to magnesium ion
response to drug
negative regulation of cytosolic calcium ion concentration
response to calcium ion
cytosolic calcium ion transport
manganese ion transmembrane transport
positive regulation of ryanodine-sensitive calcium-release channel activity by adrenergic receptor signaling pathway involved in positive regulation of cardiac muscle contraction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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