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Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (VE121G9) (Product# MA3-912) shows staining in Hela Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase (Product# MA3-912) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Human , Mouse , Rat , Dog , Rabbit , Amphibian|
|Published species reactivity||Dog , Rabbit , Porcine , Rat , Amphibian , Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified rabbit skeletal muscle sarcoplasmic reticulum.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-912 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase in canine, amphibian, human, mouse, rat, and rabbit tissues.
MA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in mouse muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-912 results in strong labeling of the entire type II (fast) myofiber. MA3-912 has also been shown to inhibit the crystallization of SERCA ATPase induced by vanadate.
The MA3-912 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium ATPases (SERCA). The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners.
MA3-912 was used in western blot to study the effects of speed endurance training and heavy resistance training on running performance improvement
|Skovgaard C,Christensen PM,Larsen S,Andersen TR,Thomassen M,Bangsbo J||Journal of applied physiology (Bethesda, Md. : 1985) (117:1097)||2014|
Four weeks of normobaric "live high-train low" do not alter muscular or systemic capacity for maintaining pH and K? homeostasis during intense exercise.
MA3-912 was used in western blot to study the effects on systemic and muscular pH control capacity and K(+) homeostasis of four weeks of normobaric hypoxia
|Nordsborg NB,Siebenmann C,Jacobs RA,Rasmussen P,Diaz V,Robach P,Lundby C||Journal of applied physiology (Bethesda, Md. : 1985) (112:2027)||2012|
Heat-shock proteins attenuate SERCA inactivation by the anti-apoptotic protein Bcl-2: possible implications for the ER Ca2+-mediated apoptosis.
MA3-912 was used in western blot to study the role of heat shock proteins in modulating the interaction between Bcl-2 and SERCA2b and the potential significance for apoptosis
|Dremina ES,Sharov VS,Sch?neich C||The Biochemical journal (444:127)||2012|
Endurance training decreases the non-linearity in the oxygen uptake-power output relationship in humans.
MA3-912 was used in western blot to investigate the effect of endurance training on muscle metabolic stability
|Majerczak J,Korostynski M,Nieckarz Z,Szkutnik Z,Duda K,Zoladz JA||Experimental physiology (97:386)||2012|
Variation in expression of calcium-handling proteins is associated with inter-individual differences in mechanical performance of rat (Rattus norvegicus) skeletal muscle.
MA3-912 was used in western blot to study the role of calcium-handling proteins in the mechanical performance of rat skeletal muscle
|James RS,Walter I,Seebacher F||The Journal of experimental biology (214:3542)||2011|
Identification of secondary effects of hyperexcitability by proteomic profiling of myotonic mouse muscle.
MA3-912 was used in western blot to identify myotonia-specific changes in protein expression in several mouse models
|Staunton L,Jockusch H,Wiegand C,Albrecht T,Ohlendieck K||Molecular bioSystems (7:2480)||2011|
Proteomic profiling of naturally protected extraocular muscles from the dystrophin-deficient mdx mouse.
MA3-912 was used in western blot to conduct proteomic profiling of extraocular muscles in Duchenne muscular dystrophy mouse model
|Lewis C,Ohlendieck K||Biochemical and biophysical research communications (396:1024)||2010|
Training effects on skeletal muscle calcium handling in human chronic heart failure.
MA3-912 was used in western blot to study the exercise-related skeletal muscle calcium homeostasis in normal and diseased hearts
|Munkvik M,Rehn TA,Slettal?kken G,Hasic A,Hall?n J,Sjaastad I,Sejersted OM,Lunde PK||Medicine and science in sports and exercise (42:847)||2010|
Altered contractility of skeletal muscle in mice deficient in titin's M-band region.
MA3-912 was used in western blot to investigate the role of M-band region of titin in muscle contraction
|Ottenheijm CA,Hidalgo C,Rost K,Gotthardt M,Granzier H||Journal of molecular biology (393:10)||2009|
Intermolecular interactions in the mechanism of skeletal muscle sarcoplasmic reticulum Ca(2+)-ATPase (SERCA1): evidence for a triprotomer.
MA3-912 was used in western blot to study the oligomerization of skeletal muscle sarcoplasmic reticulum calcium-ATPase
|Mahaney JE,Thomas DD,Farrance IK,Froehlich JP||Biochemistry (47:13711)||2008|
Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle.
MA3-912 was used in western blot to investigate the expression of sarcalumenin and related calcium-regulatory proteins in rodent skeletal muscle during aging
|O'Connell K,Gannon J,Doran P,Ohlendieck K||Experimental gerontology (43:958)||2008|
Training induced decrease in oxygen cost of cycling is accompanied by down-regulation of SERCA expression in human vastus lateralis muscle.
MA3-912 was used in western blot to study the impact of specific endurance training program on the expression of SERCA1 and SERCA2 proteins as well as on the myosin heavy chain composition in human vastus lateralis muscle
|Majerczak J,Karasinski J,Zoladz JA||Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (59:589)||2008|
Conformational fluctuations of the Ca2+-ATPase in the native membrane environment. Effects of pH, temperature, catalytic substrates, and thapsigargin.
MA3-912 was used in western blot to study the effects of various parameters on the conformational fluctuations of the SERCA Ca(2+)-ATPase in its native membrane environment
|Inesi G,Lewis D,Toyoshima C,Hirata A,de Meis L||The Journal of biological chemistry (283:1189)||2008|
Comparative effects of a low-carbohydrate diet and exercise plus a low-carbohydrate diet on muscle sarcoplasmic reticulum responses in males.
MA3-912 was used in western blot to evaluate the effect of diet and exercise on muscle sarcoplasmic reticulum responses
|Duhamel TA,Green HJ,Perco JG,Ouyang J||American journal of physiology. Cell physiology (291:C607)||2006|
Manipulation of dietary carbohydrates after prolonged effort modifies muscle sarcoplasmic reticulum responses in exercising males.
MA3-912 was used in western blot to study the correlation between muscle glycogen concentration with SR calcium regulation during prolonged moderate-intensity cycling exercise
|Duhamel TA,Perco JG,Green HJ||American journal of physiology. Regulatory, integrative and comparative physiology (291:R1100)||2006|
Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins.
MA3-912 was used in western blot to investigate the skeletal muscle sarcoplasmic reticulum proteins through microarray analyses
|Schulz JS,Palmer N,Steckelberg J,Jones SJ,Zeece MG||Biochimica et biophysica acta (1764:1429)||2006|
Displacement of SERCA from SR lipid caveolae-related domains by Bcl-2: a possible mechanism for SERCA inactivation.
MA3-912 was used in western blot to investigate the influence of Bcl-2 on SERCA displacement and activity
|Dremina ES,Sharov VS,Sch?neich C||Biochemistry (45:175)||2006|
Metabolic and sarcoplasmic reticulum Ca2+ cycling responses in human muscle 4 days following prolonged exercise.
MA3-912 was used in western blot to investigate the metabolic and sarcoplasmic reticulum Ca2+ cycling responses in human muscle following prolonged exercise
|Duhamel TA,Green HJ,Perco JG,Ouyang J||Canadian journal of physiology and pharmacology (83:643)||2005|
The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm.
MA3-912 was used in western blot to study the role of the sodium/potassium-ATPase alpha2-subunit isoform during muscle contraction
|Radzyukevich TL,Moseley AE,Shelly DA,Redden GA,Behbehani MM,Lingrel JB,Paul RJ,Heiny JA||American journal of physiology. Cell physiology (287:C1300)||2004|
Anti-apoptotic protein Bcl-2 interacts with and destabilizes the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA).
MA3-912 was used in western blot to study the interaction between the anti-apoptotic protein Bcl-2 and the SERCA
|Dremina ES,Sharov VS,Kumar K,Zaidi A,Michaelis EK,Sch?neich C||The Biochemical journal (383:361)||2004|
Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle.
MA3-912 was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia
|Glover L,Heffron JJ,Ohlendieck K||Journal of applied physiology (Bethesda, Md. : 1985) (96:11)||2004|
Paradoxical effects of prior activity on human sarcoplasmic reticulum Ca2+-ATPase response to exercise.
MA3-912 was used in western blot to investigate the influence of exercise on SERCA function in human muscle
|Tupling AR,Green HJ,Roy BD,Grant S,Ouyang J||Journal of applied physiology (Bethesda, Md. : 1985) (95:138)||2003|
Isometric force and endurance in skeletal muscle of mice devoid of all known thyroid hormone receptors.
MA3-912 was used in western blot to investigate the isometric force and endurance in the muscles of mice deficient in TRalpha1 or TRbeta.
|Johansson C,Lunde PK,Gothe S,Lannergren J,Westerblad H||The Journal of physiology (547:789)||2003|
Atrial chamber-specific expression of sarcolipin is regulated during development and hypertrophic remodeling.
MA3-912 was used in western blot to examine the effects of development and cardiac hypertrophy on the sarcolipin expression in heart.
|Minamisawa S,Wang Y,Chen J,Ishikawa Y,Chien KR,Matsuoka R||The Journal of biological chemistry (278:9570)||2003|
Mutations of either or both Cys876 and Cys888 residues of sarcoplasmic reticulum Ca2+-ATPase result in a complete loss of Ca2+ transport activity without a loss of Ca2+-dependent ATPase activity. Role of the CYS876-CYS888 disulfide bond.
MA3-912 was used in western blot to study the exact disulfide structure of Cys876 and Cys888 of sarcoplasmic reticulum calcium-ATPase and to identify the possible role of the disulfide bond.
|Daiho T,Yamasaki K,Saino T,Kamidochi M,Satoh K,Iizuka H,Suzuki H||The Journal of biological chemistry (276:32771)||2001|
Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure.
MA3-912 was used in western blot to study the contraction and intracellular calcium handling in rat skeletal muscle
|Lunde PK,Dahlstedt AJ,Bruton JD,L?nnergren J,Thor?n P,Sejersted OM,Westerblad H||Circulation research (88:1299)||2001|
Low temperature molecular adaptation of the skeletal muscle sarco(endo)plasmic reticulum Ca2+-ATPase 1 (SERCA 1) in the wood frog (Rana sylvatica).
MA3-912 was used in western blot to investigate the low temperature molecular adaptation mechanism of the skeletal muscle SERCA 1 in the wood frog.
|Dode L,Van Baelen K,Wuytack F,Dean WL||The Journal of biological chemistry (276:3911)||2001|
Enhanced sarcoplasmic reticulum Ca(2+) release following intermittent sprint training.
MA3-912 was used in western blot to study how intermittent sprint training affects sarcoplasmic reticulum functions
|Ortenblad N,Lunde PK,Levin K,Andersen JL,Pedersen PK||American journal of physiology. Regulatory, integrative and comparative physiology (279:R152)||2000|
Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice.
MA3-912 was used in western blot to investigate the role of thyroid hormone receptor (TR)-alpha1 and -beta on skeletal muscle function
|Johansson C,L?nnergren J,Lunde PK,Vennstr?m B,Thor?n P,Westerblad H||American journal of physiology. Regulatory, integrative and comparative physiology (278:R598)||2000|
Myofibrillar disorganization characterizes myopathy of camptocormia in Parkinson's disease.
MA3-912 was used in immunohistochemistry to investigate the myofibrillar disorganization in the myopathy of camptocormia
|Wrede A,Margraf NG,Goebel HH,Deuschl G,Schulz-Schaeffer WJ||Acta neuropathologica (123:419)||2012|
Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium.
MA3-912 was used in immunohistochemistry to investigate the level of SERCA1 in dilated cardiomyopathy canine myocardium
|Summerfield N,Peters ME,Hercock CA,Mobasheri A,Young IS||Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology (12:17)||2010|
TRPC3 channels colocalize with Na+/Ca2+ exchanger and Na+ pump in axial component of transverse-axial tubular system of rat ventricle.
MA3-912 was used in immunohistochemistry to investigate the TRPC signalplex in rat ventricular myocytes
|Goel M,Zuo CD,Sinkins WG,Schilling WP||American journal of physiology. Heart and circulatory physiology (292:H874)||2007|
Direct in vivo monitoring of sarcoplasmic reticulum Ca2+ and cytosolic cAMP dynamics in mouse skeletal muscle.
MA3-912 was used in immunohistochemistry to study the dynamics of sarcoplasmic reticulum Ca2+ and cytosolic cAMP in mouse skeletal muscle
|Rudolf R,Magalh?es PJ,Pozzan T||The Journal of cell biology (173:187)||2006|
Effect of chronic obstructive pulmonary disease on calcium pump ATPase expression in human diaphragm.
MA3-912 was used in immunohistochemistry and western blot to study the expression pattern of the calcium pump ATPase in human diaphragm in COPD
|Nguyen T,Rubinstein NA,Vijayasarathy C,Rome LC,Kaiser LR,Shrager JB,Levine S||Journal of applied physiology (Bethesda, Md. : 1985) (98:2004)||2005|
Serca1, SERCA1, ATP2A3, ATP2A, SERCA1a
SR Ca(2+)-ATPase 1, calcium pump 1, calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform, endoplasmic reticulum class 1/2 Ca(2+) ATPase, sarcoplasmic/endoplasmic reticulum calcium ATPase 1, ATPase, Ca++ transporting, ubiquitous, SERCA1, Sarcoplasmic or Endoplasmic Reticulum Calcium ATPase 1