Invitrogen

SGK1 Recombinant Rabbit Monoclonal Antibody (SC05-71)

View all (23) SGK1 antibodies

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Datasheet

Protocols

Questions & Answers

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SGK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of SGK1 in paraffin-embedded mouse kidney tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with SGK1 Recombinant Monoclonal Antibody (Product # MA5-41147) at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

SGK1 Antibody in Western Blot (WB) — Western Blot analysis of SGK1 in human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody SGK1 Recombinant Monoclonal Antibody (Product # MA5-41147) was used at a dilution of 1:500 in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

SGK1 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of SGK1 in 293 cells. The cells were fixed, permeabilized and stained with SGK1 Recombinant Monoclonal Antibody (Product # MA5-41147) at a dilution of 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

MA5-41147

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Expression System	HEK293 cells
Class	Recombinant Monoclonal
Type	Antibody
Clone	SC05-71
Immunogen	Synthetic peptide within human SGK1 aa 40-80 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2898901

Target Information

Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1. 3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: serine/threonine protein kinase SGK; Serine/threonine-protein kinase Sgk1; serum and glucocorticoid-dependent protein kinase; Serum/glucocorticoid-regulated kinase 1; Sgk1 variant i3

Gene Aliases: SGK; SGK1

UniProt ID: (Human) B7UUP7, (Mouse) Q9WVC6

Entrez Gene ID: (Human) 6446, (Mouse) 20393, (Rat) 29517

Molecular Function: protein modifying enzyme