Proteintech

SIX2 Monoclonal Antibody (1F9G11)

View all (8) SIX2 antibodies

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Questions & Answers

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SIX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SIX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 66347-1-IG (SIX2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SIX2 Antibody in Western Blot (WB) — Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX2 Antibody in Western Blot (WB) — Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-IG (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SIX2 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SIX2 Antibody in Western Blot (WB) — Various cells were subjected to SDS PAGE followed by western blot with 66347-1-IG (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SIX2 Antibody in Western Blot (WB) — MDCK cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

66347-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-

Product Specifications
Species Reactivity	Dog, Human, Mouse, Pig, Rat
Published species	Mouse
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	1F9G11
Immunogen	SIX2 Fusion Protein Ag2124 (1-291 aa encoded by BC024033) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSMLPTFGF TQEQVACVCE VLQQGGNIER LGRFLWSLPA CEHLHKNESV LKAKAVVAFH RGNFRELYKI LESHQFSPHN HAKLQQLWLK AHYIEAEKLR GRPLGAVGKY RVRRKFPLPR SIWDGEETSY CFKEKSRSVL REWYAHNPYP SPREKRELTE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN NENSNSNSHN PLNGSGKSVL GSSEDEKTPS GTPDHSSSSP ALLLSPPPPG LPSLHSLGHP PGPSAVPVPV PGGGGADPLQ HHHGLQDSIL NPMSANLVDL GS (1-291 aa encoded by BC024033)

Target Information

This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. SIX2 is a transcription factor which, like other members of this gene family, may be involved in limb or eye development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf.

Developmental biology

Magella B,Adam M,Potter AS,Venkatasubramanian M,Chetal K,Hay SB,Salomonis N,Potter SS

Thu Feb 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Homeobox protein SIX2; Sine oculis homeobox homolog 2; sine oculis-related homeobox 2 homolog

Gene Aliases: SIX2

UniProt ID: (Human) Q9NPC8, (Mouse) Q62232

Entrez Gene ID: (Pig) 100525814, (Human) 10736, (Dog) 481357, (Mouse) 20472, (Rat) 366542

Molecular Function: homeodomain transcription factor

kidney development chondrocyte differentiation mesenchymal to epithelial transition involved in metanephros morphogenesis transcription from RNA polymerase II promoter protein import into nucleus mesodermal cell fate specification cell proliferation anatomical structure morphogenesis anterior/posterior axis specification cell migration regulation of ossification regulation of chondrocyte differentiation middle ear morphogenesis negative regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis nephron development nephron morphogenesis mesenchymal stem cell maintenance involved in nephron morphogenesis mesenchymal cell differentiation involved in kidney development regulation of branching involved in ureteric bud morphogenesis mesenchymal stem cell proliferation positive regulation of chondrocyte proliferation metanephros development regulation of transcription, DNA-templated multicellular organism development embryonic skeletal system morphogenesis

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein complex binding DNA binding protein binding sequence-specific DNA binding