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FIGURE: 1 / 4
SLC4A4 (NBC1) (extracellular) Antibody (ANT-075-200UL) in ICC/IF
Expression of NBC1 in rat PC12 cells - Cell surface detection of NBC1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075), (1:100), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live image of the cells. C. Merge of the two images.
Product Details
ANT-075-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)KE(S)VKFGGKLVGNN, corresponding to amino acid residues 672-685 of rat NBCe1, 2ndextracellular loop, based on the 10 transmembrane topology.
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Slc4a4 encodes a sodium bicarbonate cotransporter (NBC) involved in the regulation of bicarbonate secretion and absorption and intracellular pH. Slc4a4 is an electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. Moreover, Slc4a4 may regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. Isoform 2: May have a higher activity than isoform 1. Mutations in the Slc4a4 gene are associated with proximal renal tubular acidosis. Multiple transcript variants encoding different isoforms have been found for the Slc4a4 gene. Diseases associated with SLC4A4 include Renal Tubular Acidosis, Proximal, With Ocular Abnormalities and Proximal Renal Tubular Acidosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Electrogenic sodium bicarbonate cotransporter 1; electrogenic sodium bicarbonate cotransporter NBCe1 variant D; HNBC 1; kNBC 1; Na(+)/HCO3(-) cotransporter; Na+HCO3- cotransporter 4; NBC 1; NBC 2; NBC-like protein; NBCE 1; OTTHUMP00000160355; OTTHUMP00000218884; OTTHUMP00000218885; pancreas sodium bicarbonate cotransporter; Sodium bicarbonate cotransporter; solute carrier family 4 (anion exchanger), member 4; Solute carrier family 4 member 4; solute carrier family 4, member 4; solute carrier family 4, sodium bicarbonate cotransporter, member 4
Gene Aliases: AI835705; HHNMC; HNBC1; KNBC; NBC; NBC1; NBC2; Nbc4; Nbce1; PNBC; Rnbc1; Slc4a4; SLC4A5
UniProt ID: (Mouse) O88343, (Rat) Q9JI66
Entrez Gene ID: (Mouse) 54403, (Rat) 84484
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