|Tested species reactivity||Bovine, Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Tissue / cell preparation (crude bone extract containing native osteonectin).|
|Storage buffer||50% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Radioimmune Assays (RIA)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21420 detects SPARC in Human, Rat and Bovine samples.
MA1-21420 has been successfully used in ELISA, immunohistochemistry (paraffin), radioimmune assay, and Western blot procedures.
MA1-21420 immunogen corresponds to Tissue / cell preparation (crude bone extract containing native osteonectin).
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Affinity constant: 5 x 10^9 M
SPARC is a secreted protein, acidic and rich in cysteines. It is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.