Invitrogen

SPTBN1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (16) SPTBN1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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SPTBN1 Antibody in Western Blot (WB) — Western blot analysis of Spectrin beta-1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970).

SPTBN1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Spectrin beta-1 in human lung and pancreas tissues using Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970). Corresponding SPTBN1 RNA-seq data are presented for the same tissues.

SPTBN1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of Spectrin beta-1 in human cell line A-431 using a Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970) shows localization to the Golgi apparatus.

SPTBN1 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of Spectrin beta-1 demonstrates antibody specificity. Immunohistochemical analysis of Spectrin beta-1 using anti-Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970), shows significant staining of Spectrin beta-1 in human lung and shows minimal or weak staining in human pancreas tissues. The relative expression levels of Spectrin beta-1 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (IHC)

1:200-1:500

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Spectrin beta-1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2647832

Product Specific Information

Immunogen sequence: PLATSTDHGH NLQTVQLLIK KNQTLQKEIQ GHQPRIDDIF ERSQNIVTDS SSLSAEAIRQ RLADLKQLWG LLIEETEKRH RRLEEAHRAQ QYYFDAAEAE AWMSEQEL

Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.

Target Information

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e. g. beta-1 epsilon-1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta fodrin; beta II spectrin-like (short form); beta-fodrin; beta-G spectrin; Beta-II spectrin; beta-spectrin 2; beta-spectrin 2, non-erythrocytic; beta-spectrin II; beta-spectrin non-erythrocytic 1; brain spectrin; bSPII-; cytoskeleton protein; embryonic liver beta-fodrin; Embryonic liver fodrin; embryonic liver fodrin beta chain; epididymis luminal protein 102; Fodrin beta chain; non-erythrocytic; non-erythroid spectrin beta; short form of beta II spectrin; spectrin beta 2; spectrin beta chain, brain 1; Spectrin beta chain, non-erythrocytic 1; spectrin G; spectrin, beta, non-erythrocytic 1; Spectrin, non-erythroid beta chain 1

Gene Aliases: 9930031C03Rik; AL033301; betaSpII; ELF; elf1; elf3; HEL102; mKIAA4049; Spnb-2; Spnb1; Spnb2; SPTB2; SPTBN1

UniProt ID: (Human) Q01082, (Mouse) Q62261

Entrez Gene ID: (Human) 6711, (Rat) 305614, (Mouse) 20742

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein