Invitrogen

SPTBN1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (15) SPTBN1 antibodies

Datasheet

Datasheet

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SPTBN1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of Spectrin beta-1 in human cell line A-431 using a Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970) shows localization to the Golgi apparatus.

SPTBN1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Spectrin beta-1 in human lung and pancreas tissues using Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970). Corresponding SPTBN1 RNA-seq data are presented for the same tissues.

SPTBN1 Antibody in Western Blot (WB) — Western blot analysis of Spectrin beta-1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970).

SPTBN1 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of Spectrin beta-1 demonstrates antibody specificity. Immunohistochemical analysis of Spectrin beta-1 using anti-Spectrin beta-1 Polyclonal Antibody (Product # PA5-52970), shows significant staining of Spectrin beta-1 in human lung and shows minimal or weak staining in human pancreas tissues. The relative expression levels of Spectrin beta-1 within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Spectrin beta-1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2647832

Product Specific Information

Immunogen sequence: PLATSTDHGH NLQTVQLLIK KNQTLQKEIQ GHQPRIDDIF ERSQNIVTDS SSLSAEAIRQ RLADLKQLWG LLIEETEKRH RRLEEAHRAQ QYYFDAAEAE AWMSEQEL

Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.

Target Information

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: a--fodrin; alpha II spectrin; alpha-fodrin; Alpha-II spectrin; alpha-spectrin 2; beta fodrin; beta-fodrin; beta-G spectrin; Beta-II spectrin; beta-spectrin 2; beta-spectrin 2, non-erythrocytic; beta-spectrin II; beta-spectrin non-erythrocytic 1; brain alpha-spectrin; brain spectrin; embryonic liver beta-fodrin; Embryonic liver fodrin; epididymis luminal protein 102; Fodrin alpha chain; Fodrin beta chain; inhibitory protein factor; noerythroid alpha-spectrin 2; non-erythrocytic; nonerythroid alpha-spectrin 2; spectrin alpha chain, brain; Spectrin alpha chain, non-erythrocytic 1; spectrin beta 2; spectrin beta chain, brain 1; Spectrin beta chain, non-erythrocytic 1; spectrin G; spectrin, beta, non-erythrocytic 1; spectrin, non-erythroid alpha chain; Spectrin, non-erythroid beta chain 1

Gene Aliases: 9930031C03Rik; A2a; AL033301; betaSpII; ELF; elf1; elf3; HEL102; IPF; mKIAA4049; Spna2; Spnb-2; Spnb2; Spta2; Sptan1; SPTB2; SPTBN1

UniProt ID: (Human) Q01082, (Rat) P16086, (Mouse) Q62261

Entrez Gene ID: (Human) 6711, (Rat) 64159, (Mouse) 20742

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein