Invitrogen

SQSTM1 Polyclonal Antibody

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SQSTM1 Antibody — FIGURE 3 Representative pictures of effect of I/R on DNA damage, cell survival, and inflammation. (A) DNA gel electrophoresis diagram. (B) The phosphorylation of H2AX in response to I/R-induced DNA damage ( *** P < 0.001 vs. control). Western blot analysis expression level of LC3 I/II ( * P < 0.05) (C) , Beclin1 ( *** P < 0.001), SQSTM1 ( * P < 0.05), and ATG7 ( ** P < 0.01) (D) and lysosomal associated proteins Lamp1 ( ** P < 0.01), Lamp2 ( * P < 0.05), and Cathepsin B ( * P < 0.05) (E) in total protein extract from RVA of I/R treated vs. control group. (F) The expression of NLRP3 ( *** P < 0.001), Caspase-1 ( ** P < 0.01), ASC ( ** P < 0.01), and IL-1beta ( ** P < 0.01). Results represent mean +- SE ( n = 3). The graph shows the densitometry quantification of western blot bands. Here control group represented Sham group. * P < 0.05, ** P < 0.01, and *** P < 0.001.

SQSTM1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of rat spleen cells using a SQSTM1 polyclonal antibody (Product # PA5-20839) at a 20 µg/mL dilution.

SQSTM1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of rat spleen tissue using a SQSTM1 polyclonal antibody (Product # PA5-20839) at a 5 µg/mL dilution.

SQSTM1 Antibody in Western Blot (WB) — Western blot analysis of SQSTM1 was performed by loading 20 µg of the indicated wild type (WT) or knock-out (KO) cell lines or tissues per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk in TBST for 30 minutes at room temperature. The membrane was probed with a SQSTM1 polyclonal antibody (Product # PA5-20839) at a dilution of 1:6000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG F (ab')2 secondary antibody (Product # 31461) at a dilution of 1:3000 for one hour at room temperature. Detection was performed using ECL Substrate (Product # 32106). Data courtesy of the Innovators Program.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

20 µg/mL

Immunofluorescence (IF)

20 µg/mL

Immunohistochemistry (IHC)

2-5 µg/mL

Western Blot (WB)

0.5-1 µg/mL

Published Applications

Western Blot (WB)

See 3 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 14 amino acid peptide from near the carboxy terminus of human SQSTM1.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_11157045

Product Specific Information

A suggested positive control is human spleen tissue lysate.

PA5-20839 can be used with blocking peptide PEP-0953.

This antibody has been tested by Western Blot in human and mouse samples; immunofluorescence and immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Target Information

SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappa-B by TNF-a, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A170, OSIL, PDB3, ZIP3, p60, p62, p62B; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced; oxidative stress induced like; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome-1; STONE14; Ubiquitin-binding protein p62

Gene Aliases: A170; FTDALS3; ORCA; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; SQSTM1; STAP; STONE14; ZIP; ZIP3

UniProt ID: (Human) Q13501, (Rat) O08623, (Mouse) Q64337

Entrez Gene ID: (Human) 8878, (Rat) 113894, (Mouse) 18412

Function(s)