Invitrogen

SQSTM1 Polyclonal Antibody

SQSTM1 Antibody in Immunocytochemistry (ICC/IF)

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SQSTM1 Antibody (PA5-20839) in ICC/IF

Immunofluorescence analysis of SQSTM1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SQSTM1 Polyclonal Antibody (Product # PA5-20839) at 20 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (... View More

SQSTM1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

SQSTM1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

SQSTM1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-20839

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

20 µg/mL

Immunocytochemistry (ICC/IF)

20 µg/mL

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rodent

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 13 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1. The immunogen is located within the last 50 amino acids of SQSTM1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157045

Product Specific Information

Suggested positive controls are mouse skeletal muscle, mouse pancreas, or rat liver tissue lysates.

PA5-20839 can be used with blocking peptide PEP-0953.

This antibody has been tested by Western Blot in human and mouse samples; immunofluorescence and immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Target Information

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of doxorubicin on autophagy in fibroblasts.

Human & experimental toxicology

Shuey A,Patricelli C,Oxford JT,Pu X

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Fri Feb 09 00:00:00 UTC 2024

Computational and Experimental Approaches Exploring the Role of Hesperetin in Improving Autophagy in Rat Diabetic Retinopathy.

Biomedicines

Alshaman R,Kolieb E,El-Sayed RM,Gouda SG,Alattar A,Zaitone SA,Abdelmaogood AKK,Elabbasy LM,Eltrawy AH,Sayd FY,Mokhtar HI,Wafa EGAE,Ahmed ES,Liang D,Ali DA

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Fri Mar 01 00:00:00 UTC 2024

Curcumin affects autophagy of prolactinoma cells by upregulating miR-206 to exert antitumor effects.

Journal of biochemical and molecular toxicology

Duan JF,Zhang QJ,Zhu J,Lu JH

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Sat Jun 01 00:00:00 UTC 2024

Regulation of Benzo[a]pyrene-Induced Hepatic Lipid Accumulation through CYP1B1-Induced mTOR-Mediated Lipophagy.

International journal of molecular sciences

Bu KB,Kim M,Shin MK,Lee SH,Sung JS

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Mon Jan 22 00:00:00 UTC 2024

Therapeutic effects on the development of heart failure with preserved ejection fraction by the sodium-glucose cotransporter 2 inhibitor dapagliflozin in type 2 diabetes.

Diabetology & metabolic syndrome

Feng B,Yu P,Yu H,Qian B,Li Y,Sun K,Shi B,Zhang N,Xu G

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Thu Jun 29 00:00:00 UTC 2023

Advances on the early cellular events occurring upon exposure of human macrophages to aluminum oxyhydroxide adjuvant.

Scientific reports

Masson JD,Badran G,Domdom MA,Gherardi RK,Mograbi B,Authier FJ,Crépeaux G

PA5-20839 was used in Western Blotting to investigate the early in vitro response of primary human PBMCs obtained from healthy individuals to aluminum oxyhydroxide (the most commonly used adjuvant) and a whole vaccine, in terms of internalization, conventional and non-conventional autophagy pathways, inflammation, ROS production, and mitochondrial metabolism.

Thu Feb 23 00:00:00 UTC 2023

HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy.

Cell death & disease

Cristofani R,Piccolella M,Montagnani Marelli M,Tedesco B,Poletti A,Moretti RM

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Fri Nov 18 00:00:00 UTC 2022

HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy.

Cell death & disease

Cristofani R,Piccolella M,Montagnani Marelli M,Tedesco B,Poletti A,Moretti RM

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Fri Nov 18 00:00:00 UTC 2022

NBR1 is a critical step in the repression of thermogenesis of p62-deficient adipocytes through PPARγ.

Nature communications

Huang J,Linares JF,Duran A,Xia W,Saltiel AR,Müller TD,Diaz-Meco MT,Moscat J

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Mon May 17 00:00:00 UTC 2021

NBR1 is a critical step in the repression of thermogenesis of p62-deficient adipocytes through PPARγ.

Nature communications

Huang J,Linares JF,Duran A,Xia W,Saltiel AR,Müller TD,Diaz-Meco MT,Moscat J

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Mon May 17 00:00:00 UTC 2021

Aggresome-Like Formation Promotes Resistance to Proteotoxicity in Cells from Long-Lived Species.

The journals of gerontology. Series A, Biological sciences and medical sciences

Sunchu B,Riordan RT,Yu Z,Almog I,Dimas-Munoz J,Drake AC,Perez VI

PA5-20839 was used in Immunocytochemistry to suggest that the resistance to proteotoxicity observed in long-lived species is not due to a lower level of protein aggregates but rather to enhanced handling of the protein aggregates through the formation of aggresome-like inclusions, a well-recognized protective mechanism against proteotoxicty.

Mon Jul 13 00:00:00 UTC 2020

Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma.

Oncology reports

An YW,Liu HQ,Zhou ZQ,Wang JC,Jiang GY,Li ZW,Wang F,Jin HT

PA5-20839 was used in Western Blotting to explore the antitumor effects of sinoporphyrin sodium (DVDMS)‚mediated photodynamic therapy (PDT) and sonodynamic therapy (SDT) in glioma, and to reveal the underlying mechanisms.

Thu Oct 01 00:00:00 UTC 2020

Long Term Pharmacological Perturbation of Autophagy in Mice: Are HCQ Injections a Relevant Choice?

Biomedicines

Masson JD,Blanchet B,Periou B,Authier FJ,Mograbi B,Gherardi RK,Crépeaux G

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Sun Mar 01 00:00:00 UTC 2020

Long Term Pharmacological Perturbation of Autophagy in Mice: Are HCQ Injections a Relevant Choice?

Biomedicines

Masson JD,Blanchet B,Periou B,Authier FJ,Mograbi B,Gherardi RK,Crépeaux G

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Sun Mar 01 00:00:00 UTC 2020

δ-Tocotrienol induces apoptosis, involving endoplasmic reticulum stress and autophagy, and paraptosis in prostate cancer cells.

Cell proliferation

Fontana F,Moretti RM,Raimondi M,Marzagalli M,Beretta G,Procacci P,Sartori P,Montagnani Marelli M,Limonta P

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Wed May 01 00:00:00 UTC 2019

δ-Tocotrienol induces apoptosis, involving endoplasmic reticulum stress and autophagy, and paraptosis in prostate cancer cells.

Cell proliferation

Fontana F,Moretti RM,Raimondi M,Marzagalli M,Beretta G,Procacci P,Sartori P,Montagnani Marelli M,Limonta P

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Wed May 01 00:00:00 UTC 2019

δ-Tocotrienol induces apoptosis, involving endoplasmic reticulum stress and autophagy, and paraptosis in prostate cancer cells.

Cell proliferation

Fontana F,Moretti RM,Raimondi M,Marzagalli M,Beretta G,Procacci P,Sartori P,Montagnani Marelli M,Limonta P

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Wed May 01 00:00:00 UTC 2019

δ-Tocotrienol induces apoptosis, involving endoplasmic reticulum stress and autophagy, and paraptosis in prostate cancer cells.

Cell proliferation

Fontana F,Moretti RM,Raimondi M,Marzagalli M,Beretta G,Procacci P,Sartori P,Montagnani Marelli M,Limonta P

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Wed May 01 00:00:00 UTC 2019

The Second Case of Saposin A Deficiency and Altered Autophagy.

JIMD reports

Kose M,Demir SA,Akinci G,Eraslan C,Yilmaz U,Ceylaner S,Yildirim ES,Seyrantepe V

PA5-20839 was used in Immunocytochemistry-immunoflourescence to report a case of Saposin A deficiency determined by a previously undescried homozygous variant in the saposin A domain of the PSAP gene.

Mon Sep 28 00:00:00 UTC 2020

Attenuation of autophagy flux by 6-shogaol sensitizes human liver cancer cells to TRAIL-induced apoptosis via p53 and ROS.

International journal of molecular medicine

Nazim UM,Park SY

PA5-20839 was used in Immunocytochemistry to demonstrate that 6‚shogaol (6‚sho), a bioactive component of ginger, exerted anti‚inflammatory and anticancer properties, attenuated tumor cell propagation and induced TRAIL‚mediated cell death in liver cancer cells.

Fri Feb 01 00:00:00 UTC 2019

Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.

Frontiers in pharmacology

Fauzia E,Barbhuyan TK,Shrivastava AK,Kumar M,Garg P,Khan MA,Robertson AAB,Raza SS

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Western Blot

Thu Oct 01 00:00:00 UTC 2020

Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1^H46R-expressing ALS mouse model.

Molecular brain

Mitsui S,Otomo A,Nozaki M,Ono S,Sato K,Shirakawa R,Adachi H,Aoki M,Sobue G,Shang HF,Hadano S

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Tue May 29 00:00:00 UTC 2018

Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1^H46R-expressing ALS mouse model.

Molecular brain

Mitsui S,Otomo A,Nozaki M,Ono S,Sato K,Shirakawa R,Adachi H,Aoki M,Sobue G,Shang HF,Hadano S

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Tue May 29 00:00:00 UTC 2018

Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1^H46R-expressing ALS mouse model.

Molecular brain

Mitsui S,Otomo A,Nozaki M,Ono S,Sato K,Shirakawa R,Adachi H,Aoki M,Sobue G,Shang HF,Hadano S

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Tue May 29 00:00:00 UTC 2018

Dual role of autophagy on docetaxel-sensitivity in prostate cancer cells.

Cell death & disease

Cristofani R,Montagnani Marelli M,Cicardi ME,Fontana F,Marzagalli M,Limonta P,Poletti A,Moretti RM

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Thu Aug 30 00:00:00 UTC 2018

Dual role of autophagy on docetaxel-sensitivity in prostate cancer cells.

Cell death & disease

Cristofani R,Montagnani Marelli M,Cicardi ME,Fontana F,Marzagalli M,Limonta P,Poletti A,Moretti RM

Published figure using SQSTM1 polyclonal antibody (Product # PA5-20839) in Immunocytochemistry

Thu Aug 30 00:00:00 UTC 2018

Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis.

Journal of cell science

Mukherjee R,Chakrabarti O

PA5-20839 was used in western blot to determine how mitochondrial homeostasis is affected by ubiquitin-mediated regulation of E3 ligase GP78 by MGRN1 in trans

Mon Feb 15 00:00:00 UTC 2016

Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101.

Cell death & disease

Majumder P,Chakrabarti O

PA5-20839 was used in western blot to study the fusion between lysosomes and amphisomes/MVBs regulated by mahogunin via ubiquitination of TSG101

Thu Nov 05 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; oxidative stress induced; oxidative stress induced like; p62; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; Protein kinase C-zeta-interacting protein; Sequestosome-1; STONE14; Ubiquitin-binding protein p62

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Gene Aliases: A170; FTDALS3; ORCA; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; SQSTM1; STAP; STONE14; ZIP; ZIP3

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UniProt ID: (Human) Q13501, (Rat) O08623, (Mouse) Q64337

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Entrez Gene ID: (Human) 8878, (Rat) 113894, (Mouse) 18412

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Function(s)

protein serine/threonine kinase activity protein kinase C binding protein binding zinc ion binding protein kinase binding receptor tyrosine kinase binding ubiquitin protein ligase binding SH2 domain binding identical protein binding protein homodimerization activity ubiquitin binding K63-linked polyubiquitin binding transcription cofactor activity metal ion binding

Process(es)

positive regulation of protein phosphorylation immune system process ubiquitin-dependent protein catabolic process autophagy apoptotic process macroautophagy positive regulation of macroautophagy cell differentiation regulation of I-kappaB kinase/NF-kappaB signaling protein heterooligomerization regulation of mitophagy mitophagy protein phosphorylation response to stress protein localization regulation of mitochondrion organization endosomal transport intracellular signal transduction positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of growth of symbiont in host positive regulation of transcription from RNA polymerase II promoter regulation of Ras protein signal transduction regulation of protein complex stability mitophagy in response to mitochondrial depolarization regulation of nucleic acid-templated transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SQSTM1 Polyclonal Antibody

SQSTM1 Antibody (PA5-20839) in ICC/IF

Product Details

PA5-20839

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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