In Western blot, a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).
Suggested positive control: IMR 5 and HeLa cells..
The importance of lipids for survival is underscored when starvation occurs – cellular responses occur in major organ pathways to protect against detrimental loss of lipids. For example, lipid generation and stabilization occurs in the brain even when serious deficiencies of dietary lipid exist. Embryonic and fetal tissues also are spared the effects of lacking lipids, even at the expense of maternal health. In all of these examples, the transcription factor Sterol-Regulatory Element-Binding Protein 1 (SREBP1) is a critical contributor to effective cholesterol and fatty acid synthesis, through the PPAR-gamma and AMPK signaling pathways. During fasting and starvation, more than 40% of transcription factors in an individual are dedicated to adipogenesis. However, in certain conditions that lead to obesity, the SREBP1 transcription activity is overstimulated by elevated cofactor expression (as in the case of resistin) and the result is erroneous de novo lipogenesis in hepatocytes and plasma. Excessive lipogenesis in these tissues is then associated with dyslipidemia, atherosclerosis and abnormal HDL/LDL/VLDL ratios and commonly attributed obesity-related disease.
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Protein Aliases: ADD1; Adipocyte determination- and differentiation-dependent factor 1; bHLHd1; Class D basic helix-loop-helix protein 1; nt-SREBP1; Processed sterol regulatory element-binding protein 1; SREBP-1; SREBP-1c; SREBP1; sterol regulatory element binding protein 1; sterol regulatory element binding protein-1; sterol regulatory element binding-protein 1; Sterol regulatory element-binding protein 1; Sterol regulatory element-binding transcription factor 1
Gene Aliases: ADD-1; ADD1; BHLHD1; D630008H06; I79_010155; SREBF1; SREBP-1; SREBP-1a; SREBP-1c; SREBP1; SREBP1a; SREBP1c