Immunohistochemical analysis of SSP29 / ANP32B in paraffin embedded Human Breast tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by the application of the SSP29 / ANP32B antibody (Product # PA5-18144)(3.8µg / ml) and AP-staining.
|Tested species reactivity||Human, Mouse, Pig, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (KRKRETDDEGEDD) corresponding to the C-terminus amino acids of ANP32B|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||3-5 ug/ml|
|Western Blot (WB)||0.1-0.3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
APRIL (a proliferation-inducing ligand) is a newly identified member of the tumor necrosis factor (TNF) family. Ectopic expression of APRIL is associated with the upregulation of X-linked inhibitor of apoptosis protein (XIAP) and may act as antiapoptotic factor. Although transcripts of APRIL are of low abundance in normal tissues, high levels of mRNA are detected in transformed cell lines, and in human cancers of colon, thyroid, and lymphoid tissues in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.