Immunofluorescent analysis of STAT1 in A2058 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a STAT1 monoclonal antibody (Product # MA1-037) at a dilution of 1:20 overnight at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). STAT1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Protein expressed in 293T cell transfected with human STAT1 expression vector.|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-037X can detect STAT1 in human and monkey samples and has successfully been used in western blot, IF and immunoprecipitation applications.
Note: This antibody requires overnight incubation in a Western blot for optimal performance. Do not use this antibody in a Fast Western blotting procedure.
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells.
MA1-037X was used in western blot to analyze multidrug resistant human cancer cells and autocrime cytokine/JAK/STAT-signaling induces kynurenin synthesis
|Campia I,Buondonno I,Castella B,Rolando B,Kopecka J,Gazzano E,Ghigo D,Riganti C||PloS one (10:null)||2015|