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Immunofluorescent analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (Product# MA1-13042) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3 (Product# MA1-13042), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Mouse , Human , Rat , Primate|
|Host / Isotype||Mouse / IgG|
|Immunogen||Residues 655-770 from human recombinant protein expressed in bacteria|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1-3 ul|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1600|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-13042 detects STAT3 from human, mouse, monkey and rat samples.
MA1-13042 has been successfully used in Western blot, ChIP, immunofluorescence, immunohistochemistry (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a 88 kDa protein
corresponding to STAT3. Positive control: HepG2 cells.
The MA1-13042 antigen is a recombinant protein corresponding to residues 655-770 of human STAT3.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AW109958, 1110034C02Rik, HIES, Aprf, APRF
DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3, acute phase response factor, APRF, HIES, FLJ20882, MGC16063, STAT3, Signal transducer and activator of transcription 3