Proteintech

TBP Monoclonal Antibody (2H3B2)

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TBP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 66166-1-IG (TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

TBP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — I After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 40x lens).

TBP Antibody in Western Blot (WB) — Pig liver tissue were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

TBP Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.20ug TBP antibody (66166-1-IG, red) and control antibody (blue). Fixed with 90% MeOH.

TBP Antibody in Immunoprecipitation (IP) — IP result of anti-TBP (IP:66166-1-IG, 3ug; Detection:66166-1-IG 1:800) with HEK-293 cells lysate 1800ug.

Product Details

66166-1-IG

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:16,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:325-1:1300	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Pig, Rat
Published species	Human, Rat
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	2H3B2
Immunogen	TBP Fusion Protein Ag12383 (1-338 aa encoded by BC110341) View immunogen
Conjugate	Unconjugated CoraLite488 CoraLite555 CoraLite594
Form	Liquid
Concentration	1.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Immunogen sequence: MDQNNSLPP YAQGLASPQG AMTPGIPIFS PMMPYGTGLT PQPIQNTNSL SILEEQQRQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQAVAAA AVQQSTSQQA TQGTSGQAPQ LFHSQTLTTA PLPGTTPLYP SPMTPMTPIT PATPASESSG IVPQLQNIVS TVNLGCKLDL KTIALRARNA EYNPKRFAAV IMRIREPRTT ALIFSSGKMV CTGAKSEEQS RLAARKYARV VQKLGFPAKF LDFKIQNMVG SCDVKFPIRL EGLVLTHQQF SSYEPELFPG LIYRMIKPRI VLLIFVSGKV VLTGAKVRAE IYEAFENIYP ILKGFRKTT (1-338 aa encoded by BC110341 )

Target Information

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins, such as RHOA, RAC1, and CDC42.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Imbalance of a KLF4-miR-7 auto-regulatory feedback loop promotes prostate cancer cell growth by impairing microRNA processing.

American journal of cancer research

Wei LZ,Wang YQ,Chang YL,An N,Wang X,Zhou PJ,Zhu HH,Fang YX,Gao WQ

Thu Oct 01 00:00:00 EDT 2020

B Subunit of Human Chorionic Gonadotropin Promotes Tumor Invasion and Predicts Poor Prognosis of Early-Stage Colorectal Cancer.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Li J,Yin M,Song W,Cui F,Wang W,Wang S,Zhu H

Fri Mar 16 00:00:00 EDT 2018

Down-regulation of miR-144 elicits proinflammatory cytokine production by targeting toll-like receptor 2 in nonalcoholic steatohepatitis of high-fat-diet-induced metabolic syndrome E3 rats.

Molecular and cellular endocrinology

Li D,Wang X,Lan X,Li Y,Liu L,Yi J,Li J,Sun Q,Wang Y,Li H,Zhong N,Holmdahl R,Lu S

Sun Feb 15 00:00:00 EST 2015

Bioinformatics

Protein Aliases: MGC117320; MGC126054; MGC126055; RP1-191N21.3; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; tata-box-binding; TATA-box-binding protein; TFIID core protein; Transcription initiation factor TFIID TBP subunit

Gene Aliases: GTF2D; GTF2D1; HDL4; SCA17; TBP; TF2D; TFIID

UniProt ID: (Human) P20226, (Mouse) P29037

Entrez Gene ID: (Pig) 110259740, (Human) 6908, (Mouse) 21374, (Rat) 117526

Molecular Function: general transcription factor