Invitrogen

TEF1 Monoclonal Antibody (GT13112)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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TEF1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of TEF1 in HeLa cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with TEF1 monoclonal antibody (Product # MA5-27786) using a dilution of 1:200, followed by phalloidin.

TEF1 Antibody in Western Blot (WB) — Western blot was performed using TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) and a~48 kDa band corresponding to TEAD1 was observed across all the cell lines tested along with an uncharacterized band (*) at 30 kDa. Whole cell extracts (30 µg lysate) of A431 (Lane 1), HeLa (Lane 2), U2OS (Lane 3), BeWo (Lane 4), HEL 92.1.7 (Lane 5), U937 (Lane 6) and MOLT4 (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

TEF1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of TEF1 was performed in HeLa nuclear extracts using 5 µg of TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786). Samples were transferred to a membrane and probed with TEF1 Monoclonal Antibody (GT13112) as a primary antibody and an HRP-conjugated anti-Mouse IgG was used as a secondary antibody.

TEF1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells and tissue tested owing to their inherent genetic constitution. Relative expression of TEAD1 was observed in A431, HeLa, U2OS, BeWo and MOLT4 in comparison to HEL 92.1.7 and U937 using TEF1 Monoclonal Antibody (GT13112) (Product # MA5-27786) in Western Blot. {RE}

Product Details

MA5-27786

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	GT13112
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEF1. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735391

Product Specific Information

Positive Control: HeLa NE

Predicted Reactivity: Pig (100%), Chicken (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: N TEAD1; NTEF-1; NTEF1; Protein GT-IIC; TCF-13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEAD-1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1

Gene Aliases: AA; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEAD1; TEF-1; TEF1

UniProt ID: (Human) P28347

Entrez Gene ID: (Human) 7003

Molecular Function: DNA-binding transcription factor