Invitrogen

TEF1 Monoclonal Antibody (GT13112)

Product Image Gallery

TEF1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of TEF1 in HeLa cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with TEF1 monoclonal antibody (Product # MA5-27786) using a dilution of 1:200, followed by phalloidin.

TEF1 Antibody in Western Blot (WB) — Western blot analysis of TEF1 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with TEF1 monoclonal antibody (Product # MA5-27786) using a dilution of 1:500.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT13112

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEF1. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.66 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735391

Product Specific Information

Positive Control: HeLa NE

Predicted Reactivity: Pig (100%), Chicken (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AA; NTEF-1; Protein GT-IIC; REF1; TCF-13; TCF13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEAD-1; TEAD1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1

Gene Aliases: AA; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEAD1; TEF-1; TEF1

UniProt ID: (Human) P28347

Entrez Gene ID: (Human) 7003

Molecular Function: transcription factor