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          • Primary Antibodies ›
          • TGF beta-1,2,3 Antibodies

          Invitrogen

          TGF beta-1,2,3 Monoclonal Antibody (1D11.16.8), Functional Grade, eBioscience™

          2 Published Figures
          13 References
          View all (12) TGF beta-1,2,3 antibodies

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          Cite TGF beta-1,2,3 Monoclonal Antibody (1D11.16.8), Functional Grade, eBioscience™

          Additional Information:

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          • Published Figures (2)
          TGF beta-1,2,3 Antibody in Neutralization (Neu)
          Group 53 Created with Sketch.
          TGF beta-1,2,3 Antibody in Neutralization (Neu)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          TGF beta-1,2,3 Antibody (16-9243-85) in Neu

          Pathology oncology research : POR 2022 - FIGURE 2 Ets1 upregulation in glioblastoma endothelial cells depend on TGFbeta signaling. (A) RNA levels of Ets1 in bEND.3 cells treated with VEGFA, TNFalpha or TGFbeta (mean + SD of four independent experiments; ** p < 0.01; ANOVA with Tukey's multiple comparisons test). (B) RNA levels of Ets1 in bEND.3 cells treated with conditioned medium from CT-2A glioma cells with TGFbeta neutralizing antibody or cont... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          TGF beta-1,2,3 Antibody in Neutralization (Neu)
          TGF beta-1,2,3 Antibody in Neutralization (Neu)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          16-9243-85

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-Dependent
          -

          Flow Cytometry (Flow)

          -
          View 2 publications 2 publications

          ELISA (ELISA)

          -
          View 2 publications 2 publications

          Neutralization (Neu)

          Assay-Dependent
          View 5 publications 5 publications

          Functional Assay (Functional)

          Assay-Dependent
          -

          Inhibition Assays (Inhibition)

          -
          View 1 publication 1 publication

          T-Cell Activation (TCA)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Hamster, Human, Mouse, Non-human primate, Rhesus monkey, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1D11.16.8

          Conjugate

          Functional Grade Functional Grade Functional Grade

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          500 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2573124

          Product Specific Information

          Description: The monoclonal antibody 1D11.16.8 recognizes human, mouse, rat, hamster, canine and non-human primate Transforming Growth Factor (TGF) beta 1, 2 and 3. TGF beta is a pleiotropic cytokine that exists as five isoforms (TGF beta 1-5) that share 70-80% homology with each other. However, these isoforms are not homologous to TGF alpha. TGF beta 1 is highly conserved between human, murine, simian, bovine, porcine, and chicken proteins. TGF beta 1 is the most abundant isoform and is ubiquitously expressed by a variety of cell types, including platelets, macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. However, the other isoforms exhibit a more restricted expression pattern.

          TGF beta 1 is synthesized as a long precursor polypeptide, which is cleaved to yield the mature protein and the Latency Associated Peptide (LAP). LAP and mature TGF beta 1 remain non-covalently associated during secretion, forming homodimers known as the Small Latent Complex (SLC). Secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. The bioactivity of mature TGF beta 1 is dependent on its release from LAP by conformational changes and proteolytic processing. Its activities include inhibition of cell growth in epithelial cells, endothelial cells, fibroblasts, neurons, lymphoid cells, and other hematopoietic cell types. TGF beta 1 also inhibits the proliferation of T and NK cells, downregulates the activities of activated macrophages, and blocks the anti-tumor activity of IL-2 - bearing lymphokine-activated killer (LAK) cells. Recently, TGF beta 1 has been found to play a critical role in the development of regulatory T cells and act as a costimulatory factor for expression of Foxp3. Dendritic cells exposed to tumors have been reported to secrete TGF beta 1 and stimulate the differntiation of CD4+CD25+ Treg cell from peripheral CD4+CD25- progeny. TGF beta 1-induced regulatory T cells have been termed iTregs.

          Applications Reported: This 1D11.16.8 antibody has been reported for use in neutralization assays both in vivo and in vitro and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          TGF-beta1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta1, -2, and -3 are disulfide-linked homodimers.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: BSC-1 cell growth inhibitor; cetermin; glioblastoma-derived T-cell suppressor factor; latency-associated peptide; polyergin; prepro-transforming growth factor beta-1; prepro-transforming growth factor beta-2; prepro-transforming growth factor beta-3; tgf beta 1; TGF beta 2 protein; tgf beta 3; tgf beta1; TGF-beta precursor; TGF-beta-1; TGF-beta-2; TGF-beta-3; TGF-beta2; TGF-beta3; Transforming growth factor; Transforming growth factor beta-2; Transforming growth factor beta-3; transforming growth factor beta-3 (TGF beta-3) precursor (start site 611 could be 368 or 477); transforming growth factor beta-3 preproprotein; transforming growth factor beta-3-like protein; transforming growth factor beta1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta 2; transforming growth factor, beta 3; transforming growth factor-beta-2 precursor; transforming growth factor-beta3; unnamed protein product

          View more View less

          Gene Aliases: ARVD; ARVD1; CAEND1; CAEND2; CED; DPD1; EGK_01648; EGK_18402; G-TSF; I79_000092; IBDIMDE; LAP; LDS4; LDS5; RNHF; TGF-B2; TGF-B3; TGF-beta-3; TGF-beta1; TGF-beta2; TGF-beta3; TGFB; Tgfb-1; Tgfb-2; Tgfb-3; TGFbeta; TGFbeta1; TGFbeta2; TGFbeta3

          View more View less

          UniProt ID: (Human) P01137, (Human) P61812, (Human) P10600, (Mouse) P04202, (Rat) P17246, (Mouse) P27090, (Rat) Q07257, (Rat) Q07258

          View more View less

          Entrez Gene ID: (Human) 7040, (Dog) 403998, (Human) 7042, (Dog) 488596, (Rhesus monkey) 707540, (Human) 7043, (Dog) 490796, (Rhesus monkey) 703239, (Chinese hamster) 100773359, (Mouse) 21803, (Rat) 59086, (Mouse) 21808, (Chinese hamster) 100774918, (Rat) 81809, (Rat) 25717, (Mouse) 21809, (Chinese hamster) 100756831

          View more View less

          Function(s)
          type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding identical protein binding protein serine/threonine kinase activator activity macromolecular complex binding antigen binding beta-amyloid binding receptor binding protein homodimerization activity transforming growth factor beta binding deubiquitinase activator activity growth factor
          Process(es)
          vasculogenesis ureteric bud development response to hypoxia morphogenesis of a branching structure epithelial to mesenchymal transition neural tube closure sprouting angiogenesis chondrocyte differentiation hematopoietic progenitor cell differentiation connective tissue replacement involved in inflammatory response wound healing adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target heart valve morphogenesis aortic valve morphogenesis protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process apoptotic process transforming growth factor beta receptor signaling pathway I-kappaB kinase/NF-kappaB signaling negative regulation of neuroblast proliferation salivary gland morphogenesis heart development female pregnancy positive regulation of cell proliferation negative regulation of cell proliferation response to xenobiotic stimulus response to wounding response to glucose response to gamma radiation positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of extracellular matrix disassembly positive regulation of epithelial to mesenchymal transition macrophage derived foam cell differentiation positive regulation of fibroblast migration negative regulation of macrophage cytokine production oligodendrocyte development positive regulation of microglia differentiation regulation of striated muscle tissue development neural tube development cell differentiation hyaluronan catabolic process negative regulation of cell growth regulation of cell migration positive regulation of cell migration organ regeneration membrane protein intracellular domain proteolysis positive regulation of protein complex assembly positive regulation of exit from mitosis response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production receptor catabolic process positive regulation of superoxide anion generation positive regulation of collagen biosynthetic process response to vitamin D negative regulation of MyD88-dependent toll-like receptor signaling pathway response to laminar fluid shear stress response to immobilization stress cellular response to platelet-derived growth factor stimulus myofibroblast differentiation regulation of cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth myelination positive regulation of apoptotic process positive regulation of vascular permeability positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration regulatory T cell differentiation cell-cell junction organization response to ethanol positive regulation of regulatory T cell differentiation negative regulation of cell differentiation positive regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation positive regulation of epidermal growth factor receptor signaling pathway negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgA isotypes lymph node development digestive tract development negative regulation of skeletal muscle tissue development positive regulation of smooth muscle cell proliferation inner ear development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of inflammatory response negative regulation of phagocytosis defense response to fungus positive regulation of chemotaxis positive regulation of cellular component organization positive regulation of multicellular organismal process positive regulation of protein metabolic process negative regulation of release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling ventricular cardiac muscle tissue morphogenesis regulation of blood vessel remodeling face morphogenesis frontal suture morphogenesis positive regulation of SMAD protein import into nucleus negative regulation of biomineral tissue development positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to mechanical stimulus cellular response to glucose stimulus cellular response to growth factor stimulus cellular response to low-density lipoprotein particle stimulus cellular response to hypoxia cellular response to ionizing radiation cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus positive regulation of mononuclear cell migration odontoblast differentiation extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis positive regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway liver regeneration cellular response to virus transforming growth factor beta receptor superfamily signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of protein localization to nucleus positive regulation of extracellular matrix assembly response to salt positive regulation of mesenchymal stem cell proliferation regulation of miRNA transcription negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane positive regulation of vasculature development positive regulation of STAT cascade regulation of epithelial to mesenchymal transition involved in endocardial cushion formation cellular response to acetaldehyde cellular response to insulin-like growth factor stimulus embryonic liver development negative regulation of cell-cell adhesion mediated by cadherin positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of endothelial cell apoptotic process positive regulation of primary miRNA processing positive regulation of cardiac muscle cell differentiation MAPK cascade negative regulation of protein phosphorylation positive regulation of protein phosphorylation inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation cell migration regulation of transforming growth factor beta receptor signaling pathway negative regulation of cell-cell adhesion positive regulation of bone mineralization lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process positive regulation of peptidyl-serine phosphorylation positive regulation of protein dephosphorylation positive regulation of MAP kinase activity protein kinase B signaling positive regulation of phosphatidylinositol 3-kinase activity ossification involved in bone remodeling negative regulation of mitotic cell cycle positive regulation of cell division pathway-restricted SMAD protein phosphorylation regulation of SMAD protein import into nucleus cellular response to organic cyclic compound positive regulation of NAD+ ADP-ribosyltransferase activity positive regulation of transcription regulatory region DNA binding cell morphogenesis skeletal system development eye development kidney development hair follicle development heart morphogenesis outflow tract septum morphogenesis membranous septum morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis cardiac right ventricle morphogenesis ventricular trabecula myocardium morphogenesis endocardial cushion fusion atrial septum primum morphogenesis neural retina development sensory organ development glial cell migration male gonad development embryo development ending in birth or egg hatching cardioblast differentiation positive regulation of epithelial cell migration striated muscle tissue development negative regulation of angiogenesis signaling hemopoiesis collagen fibril organization positive regulation of cell growth embryonic limb morphogenesis neutrophil chemotaxis epithelial cell differentiation hair follicle morphogenesis positive regulation of stress-activated MAPK cascade regulation of transforming growth factor beta2 production regulation of actin cytoskeleton organization positive regulation of cell adhesion mediated by integrin ascending aorta morphogenesis wound healing dopamine biosynthetic process odontogenesis uterine wall breakdown positive regulation of neuron apoptotic process positive regulation of integrin biosynthetic process positive regulation of Notch signaling pathway positive regulation of cell cycle positive regulation of heart contraction negative regulation of Ras protein signal transduction somatic stem cell division embryonic digestive tract development neuron development generation of neurons positive regulation of immune response regulation of timing of catagen positive regulation of catagen positive regulation of cardioblast differentiation cardiac muscle cell proliferation uterus development cardiac epithelial to mesenchymal transition ventricular septum morphogenesis atrial septum morphogenesis pharyngeal arch artery morphogenesis secondary palate development positive regulation of cardiac epithelial to mesenchymal transition positive regulation of extracellular matrix disassembly regulation of apoptotic process involved in outflow tract morphogenesis regulation of complement-dependent cytotoxicity substantia propria of cornea development pericyte cell differentiation cranial skeletal system development negative regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation in utero embryonic development mammary gland development negative regulation of neuron apoptotic process lung alveolus development positive regulation of filopodium assembly positive regulation of stress fiber assembly detection of hypoxia negative regulation of vascular smooth muscle cell proliferation positive regulation of occluding junction disassembly negative regulation of transcription from RNA polymerase II promoter regulation of cell growth cell activation regulation of sodium ion transport columnar/cuboidal epithelial cell maturation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment tolerance induction to self antigen protein phosphorylation cellular calcium ion homeostasis defense response Notch signaling pathway endoderm development skeletal muscle tissue development cell proliferation germ cell migration organ morphogenesis gene expression regulation of gene expression regulation of metal ion transport T cell differentiation negative regulation of ossification osteoclast differentiation regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation mononuclear cell proliferation tube development T cell activation negative regulation of T cell proliferation positive regulation of odontogenesis myeloid dendritic cell differentiation T cell homeostasis surfactant homeostasis proteasome-mediated ubiquitin-dependent protein catabolic process regulation of regulatory T cell differentiation muscle cell cellular homeostasis positive regulation of fibroblast proliferation negative regulation of T cell activation positive regulation of smooth muscle cell differentiation neuron apoptotic process regulation of cell cycle phospholipid homeostasis canonical Wnt signaling pathway respiratory system development regulation of morphogenesis of a branching structure mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis regulation of cartilage development retina vasculature development in camera-type eye connective tissue development lens fiber cell differentiation T-helper 17 cell lineage commitment regulation of morphogenesis of an epithelium regulation of organ morphogenesis lung development cartilage condensation blood vessel development blood vessel remodeling cardiac left ventricle morphogenesis axon guidance muscle organ development extracellular matrix organization regulation of apoptotic process negative regulation of apoptotic process response to estrogen negative regulation of keratinocyte differentiation neuron fate commitment embryonic neurocranium morphogenesis negative regulation of cartilage development positive regulation of activation-induced cell death of T cells regulation of extracellular matrix organization positive regulation of GTP binding positive regulation of extrinsic apoptotic signaling pathway in absence of ligand thyroid gland development hindbrain development pancreas development response to retinoic acid response to cytokine negative regulation of transforming growth factor beta receptor signaling pathway regulation of epithelial cell differentiation regulation of endothelial cell differentiation regulation of epithelial cell proliferation palate development primary palate development system development
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