Invitrogen

TGFBR2 Polyclonal Antibody

View all (21) TGFBR2 antibodies

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TGFBR2 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of TGFBR2 was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TGFBR2 Rabbit Polyclonal Antibody(Product # PA5-35076) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TGFBR2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of TGFBR2 in HepG2 cells using a TGFBR2 polyclonal antibody (Product # PA5-35076) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).

TGFBR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TGFBR2 in formalin-fixed, paraffin-embedded human placenta tissue using a TGFBR2 polyclonal antibody (Product # PA5-35076) followed by peroxidase conjugation of the secondary antibody and DAB staining.

TGFBR2 Antibody in Western Blot (WB) — Western blot analysis of TGFBR2 in mouse lung tissue lysate (35 µg/lane) using a TGFBR2 polyclonal antibody (Product # PA5-35076).

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:10-1:50

Immunofluorescence (IF)

1:10-1:50

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TGFBR2

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2552386

Target Information

This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: tbetaR-II; TGF-beta receptor II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta type II receptor; TGFBR2; TGFR-2; transforming growth factor beta receptor II; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II alpha; transforming growth factor, beta receptor II beta; transforming growth factor, beta receptor II delta; transforming growth factor, beta receptor II epsilon; transforming growth factor, beta receptor II gamma; Transforming growth factor-beta receptor type II

Gene Aliases: 1110020H15Rik; AAT3; AU042018; DNIIR; FAA3; LDS1B; LDS2; LDS2B; MFS2; RIIC; RIIDN; TAAD2; TbetaR-II; TbetaRII; TBR-II; TGFbeta-RII; TGFBR2; TGFR-2

UniProt ID: (Human) P37173, (Mouse) Q62312

Entrez Gene ID: (Human) 7048, (Mouse) 21813

Molecular Function: TGF-beta receptor cytokine receptor kinase protein kinase protein kinase receptor receptor serine/threonine protein kinase receptor transferase

blood vessel development patterning of blood vessels vasculogenesis in utero embryonic development heart looping positive regulation of mesenchymal cell proliferation lens development in camera-type eye positive regulation of tolerance induction to self antigen positive regulation of B cell tolerance induction positive regulation of T cell tolerance induction outflow tract morphogenesis growth plate cartilage chondrocyte growth protein phosphorylation receptor-mediated endocytosis apoptotic process transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation Notch signaling pathway smoothened signaling pathway gastrulation brain development heart development embryo implantation aging response to nutrient positive regulation of cell proliferation response to mechanical stimulus response to glucose regulation of gene expression positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition peptidyl-serine phosphorylation peptidyl-threonine phosphorylation signal transduction by protein phosphorylation negative regulation of transforming growth factor beta receptor signaling pathway organ regeneration activation of protein kinase activity embryonic hemopoiesis wound healing regulation of cell proliferation response to drug myeloid dendritic cell differentiation positive regulation of skeletal muscle tissue regeneration response to estrogen positive regulation of angiogenesis response to steroid hormone digestive tract development positive regulation of smooth muscle cell proliferation embryonic cranial skeleton morphogenesis positive regulation of NK T cell differentiation palate development negative regulation of cardiac muscle cell proliferation pathway-restricted SMAD protein phosphorylation ventricular septum morphogenesis bronchus morphogenesis trachea formation mammary gland morphogenesis lung lobe morphogenesis response to cholesterol positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation lens fiber cell apoptotic process positive regulation of reactive oxygen species metabolic process growth plate cartilage development transmembrane receptor protein serine/threonine kinase signaling pathway negative regulation of cell proliferation organ morphogenesis phosphorylation cell differentiation lung development regulation of growth cartilage development lung morphogenesis bronchus development trachea morphogenesis

Process(es)

transmembrane receptor protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity transforming growth factor beta-activated receptor activity transforming growth factor beta receptor activity, type II protein binding ATP binding glycosaminoglycan binding mitogen-activated protein kinase kinase kinase binding type I transforming growth factor beta receptor binding SMAD binding metal ion binding transforming growth factor beta binding nucleotide binding protein kinase activity protein serine/threonine kinase activity receptor activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups type III transforming growth factor beta receptor binding