Invitrogen

TIMP2 Monoclonal Antibody (F27 P3 A4)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

5 References

View all (68) TIMP2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

TIMP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

TIMP2 Antibody (MA1-774) in ICC/IF

Immunofluorescence analysis of TIMP2 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP2 (F27 P3 A4) Mouse Monoclonal Antibody (Product # MA1-774) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) ... View More

TIMP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TIMP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lysates tested owing to their inherent genetic constitution. Relative expression of TIMP2 was observed in HT-1080 as compared to Daudi using Anti-TIMP2 Monoclonal Antibody (F27 P3 A4) (Product # MA1-774) in Western Blot. {RE}

Product Details

MA1-774

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Plant

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

F27 P3 A4

Immunogen

Synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_889483

Product Specific Information

MA1-774 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-774 detects TIMP-2 from mouse samples.

MA1-774 has been successfully used in Western blot, IF and immunohistochemistry procedures. By Western blot, MA1-774 detects a ~18-24 kDa band representing TIMP-2 from mouse platelets. It is recommended to block with 3% BSA.

The MA1-774 immunogen is a synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2. This sequence is 100% conserved in human, rat, rabbit, chicken, and bovine TIMP-2

Target Information

TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

MA1-774 was used in Western Blot to demonstrate that the reduced dimeric form of Gal-1 is responsible for inducing improvements to muscle membrane repair, while the monomeric form is responsible for changes in inflammation.

Wed Nov 17 00:00:00 EST 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

Wed Nov 17 00:00:00 EST 2021

Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice.

Molecules (Basel, Switzerland)

Yi R,Zhang J,Sun P,Qian Y,Zhao X

MA1-774 was used in Western Blotting to study the protective effects of Kuding tea polyphenols on ultraviolet B-induced skin injury of SKH1 hairless mice.

Wed Mar 13 00:00:00 EDT 2019

A Genetic Screen Identifies PRP18a, a Putative Second Step Splicing Factor Important for Alternative Splicing and a Normal Phenotype in Arabidopsis thaliana.

G3 (Bethesda, Md.)

Kanno T,Lin WD,Chang CL,Matzke M,Matzke AJM

MA1-774 was used in Western Blotting to identify a putative second step splicing factor, important for alternative splicing and a normal phenotype in Arabidopsis thaliana.

Wed Mar 28 00:00:00 EDT 2018

CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes.

Cancer cell

Xia L,Huang W,Bellani M,Seidman MM,Wu K,Fan D,Nie Y,Cai Y,Zhang YW,Yu LR,Li H,Zahnow CA,Xie W,Chiu Yen RW,Rassool FV,Baylin SB

MA1-774 was used in Immunohistochemistry to identify that CHD4 knockdown activates silenced tumour suppressor genes, revealing their role for blunting colorectal cancer cell proliferation, invasion, and metastases.

Mon May 08 00:00:00 EDT 2017

Inhibition of Smooth Muscle β-Catenin Hinders Neointima Formation After Vascular Injury.

Arteriosclerosis, thrombosis, and vascular biology

Riascos-Bernal DF,Chinnasamy P,Gross JN,Almonte V,Egaña-Gorroño L,Parikh D,Jayakumar S,Guo L,Sibinga NES

MA1774 was used in western blot to determine the impact of smooth muscle cell beta-catenin to vascular homeostasis and arterial injury

Mon May 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CSC-21K; Metalloproteinase inhibitor 2; testicular secretory protein Li 57; TIMP 2; TIMP-2; Tissue inhibitor of metalloproteinases 2

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Gene Aliases: CSC-21K; D11Bwg1104e; DDC8; Timp-2; TIMP2

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UniProt ID: (Human) P16035

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Entrez Gene ID: (Human) 7077, (Mouse) 21858

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Function(s)

protease binding integrin binding protein binding metalloendopeptidase inhibitor activity metal ion binding enzyme inhibitor activity enzyme activator activity peptidase inhibitor activity protease inhibitor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIMP2 Monoclonal Antibody (F27 P3 A4)

TIMP2 Antibody (MA1-774) in ICC/IF

Product Details

MA1-774

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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