Western blot analysis of TLR4 in Daudi cell lysate in the 1) absence and 2) presence of immunizing peptide, 3) Ramos cell lysate, 4) human colon tumor lysate, 5) corresponding colon normal adjacent tissue lysate, 6) human colon tumor lysate and 7) corresponding colon normal adjacent tissue lysate using a CD284/TLR4/Toll-like Receptor 4 polyclonal antibody (Product # PA5-23270) . For the cell line testing, 2 ug/ml was used; for the colon lysate, 5 ug/ml was used.
|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (666 KYGRGENIYDAF 677) of TLR4 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 µg/ml|
TLR4 is an 839 amino acid member of the Toll-like receptor (TLR) family of transmembrane proteins comprised of multiple copies of leucine rich repeats in the extracellular domain and a TIR domain. It is a widely studied, phylogenetically conserved, member playing a fundamental role in pathogen recognition and activation of innate immunity. Like its counterpart in Drosophila, TLR4 signals through adaptor molecules and mediates the production of cytokines necessary for the development of effective immunity. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. This receptor is most abundantly expressed in placenta, spleen and in myelomonocytic subpopulation of the leukocytes. Defects in this protein have been associated with differences in LPS responsiveness.