Alomone Labs, LTD

TRPC4 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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TRPC4 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of TRPC4 in mouse cerebellum - Immunohistochemical staining ofmouse cerebellum frozen sections with Anti-TRPC4 Antibody (#ACC-018). A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse Anti-parvalbumin (PV)in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.

TRPC4 Antibody in Western Blot (WB) — Western blot analysis of human PC3 Caucasian prostate adenocarcinoma (lanes 1 and 3) andhuman LNCaP prostate carcinoma (lanes 2 and 4) cell lysates: - 1,3. Anti-TRPC4 Antibody (#ACC-018), (1:200).2,4. Anti-TRPC4 Antibody , preincubated with TRPC4 Blocking Peptide (#BLP-CC018).

TRPC4 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of rat brain lysate: - 1. Rat brain lysate.2.Lysate immunoprecipitated with Anti-TRPC4 Antibody (#ACC-018), (6 µg).3. Lysate immunoprecipitated with pre-immune rabbit serum. The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain. Western blot analysiswas performed with Anti-TRPC4 Antibody .

Product Details

ACC-018-200UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunoprecipitation (IP)	6 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	(C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4, Intracellular, C-terminus View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

TRPC4 is a member of the canonical subfamily of transient receptor potential cation channels. TRPC4 forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in TRPC4 may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for TRPC4. The classical or canonical transient receptor potential (TRPC) channels are the subfamily most closely related to the founding member of the TRP family, the Drosophila TRP channel. TRPC channels have seven TRPC genes that encode the channel protein (TRPC1-7). Diseases associated with TRPC4 include Photosensitive Epilepsy.CaV3.3 encodes the pore-forming alpha subunit of a voltage gated calcium channel. The encoded protein is a member of a subfamily of calcium channels referred to as is a low voltage-activated, T-type, calcium channel. The channel encoded by CaV3.3 is characterized by a slower activation and inactivation compared to other T-type calcium channels. CaV3.3 may be involved in calcium signaling in neurons. Alternate splicing results in multiple transcript variants of CaV3.3. Voltage-gated calcium channels (CaV) are present in the membrane of most excitable cells and mediate calcium influx in response to depolarization, an proteins such as CaV3.3 regulate intracellular processes such as contraction, secretion, neurotransmission and gene expression. Diseases associated with CACNA1I include Childhood Absence Epilepsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha TRPC4; beta TRPC4; beta-2 TRPC4; capacitative calcium entry channel 1; Capacitative calcium entry channel Trp4; CCE1; hTrp-4; MGC119570; MGC119571; MGC119572; MGC119573; putative capacitative calcium entry channel; Receptor-activated cation channel TRP4; Short transient receptor potential channel 4; transient receptor potential cation channel, subfamily C, member 4; transient receptor potential channel 4; transient receptor potential protein 4; transient receptor protein 4; Trp-related protein 4; TRPC 4; TrpC4; Trpc4_v2

Gene Aliases: CCE1; HTRP-4; HTRP4; STRPC4; TRP4; TRPC4; Trrp4

UniProt ID: (Human) Q9UBN4, (Mouse) Q9QUQ5

Entrez Gene ID: (Human) 7223, (Rat) 84494, (Mouse) 22066

Molecular Function: ion channel transporter