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Immunofluorescence analysis of Tau [Tau-5] was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Tau [Tau-5] Mouse Monoclonal Antibody (AHB0042) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization and panel e is a no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Mouse , Human , Rat , Cattle , Sheep|
|Published species reactivity||Rat , Cattle , Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified bovine microtubule-associated proteins.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Co-chaperone BAG2 Mediates Cold-Induced Accumulation of Phosphorylated Tau in SH-SY5Y Cells.
AHB0042 was used in western blot to test if BAG2 mediates the cold-induced accumulation of phosphorylated tau protein.
|de Paula CA,Santiago FE,de Oliveira AS,Oliveira FA,Almeida MC,Carrettiero DC||Cellular and molecular neurobiology (36:593)||2016|
Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth.
AHB0042 was used in western blot to investigate the role of Apaf1 in axonogenesis
|De Zio D,Molinari F,Rizza S,Gatta L,Ciotti MT,Salvatore AM,Mathiassen SG,Cwetsch AW,Filomeni G,Rosano G,Ferraro E||Cellular and molecular life sciences : CMLS (72:4173)||2015|
Activation of Cdk5/p25 and tau phosphorylation following chronic brain hypoperfusion in rats involves microRNA-195 down-regulation.
AHB0042 was used in western blot to investigate the involvement of microRNA-195 in the effect of chronic brain hypoperfusion on rodent Cdk5/p25 and tau phosphorylation
|Sun LH,Ban T,Liu CD,Chen QX,Wang X,Yan ML,Hu XL,Su XL,Bao YN,Sun LL,Zhao LJ,Pei SC,Jiang XM,Zong DK,Ai J||Journal of neurochemistry (134:1139)||2015|
Opposing effects of membrane-anchored CX3CL1 on amyloid and tau pathologies via the p38 MAPK pathway.
AHB0042 was used in western blot to investigate the role of membrane-anchored versus soluble CX3CL1 in regulating the microglia-mediated amelioration of Abeta pathology
|Lee S,Xu G,Jay TR,Bhatta S,Kim KW,Jung S,Landreth GE,Ransohoff RM,Lamb BT||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:12538)||2014|
Early alterations in energy metabolism in the hippocampus of APPswe/PS1dE9 mouse model of Alzheimer's disease.
AHB0042 was used in western blot to investigate the abnormalites in hippocampal energy metabolism in the pathogenesis of Alzheimer disease
|Pedrós I,Petrov D,Allgaier M,Sureda F,Barroso E,Beas-Zarate C,Auladell C,Pallàs M,Vázquez-Carrera M,Casadesús G,Folch J,Camins A||Biochimica et biophysica acta (1842:1556)||2014|
Profiling murine tau with 0N, 1N and 2N isoform-specific antibodies in brain and peripheral organs reveals distinct subcellular localization, with the 1N isoform being enriched in the nucleus.
AHB0042 was used in western blot to investigate the murine tau isoforms in brain and peripheral organs
|Liu C,Götz J||PloS one (8:null)||2014|
Cellular prion protein modulates β-amyloid deposition in aged APP/PS1 transgenic mice.
AHB0042 was used in western blot to test if β-amyloid accumulation affects prion infectivity and if different amounts of PrP affect β-amyloid accumulation.
|Ordóñez-Gutiérrez L,Torres JM,Gavín R,Antón M,Arroba-Espinosa AI,Espinosa JC,Vergara C,Del Río JA,Wandosell F||Neurobiology of aging (34:2793)||2013|
Dietary resveratrol prevents Alzheimer's markers and increases life span in SAMP8.
AHB0042 was used in western blot to elucidate the role of dietary resveratrol in SAMP8 mice.
|Porquet D,Casadesús G,Bayod S,Vicente A,Canudas AM,Vilaplana J,Pelegrí C,Sanfeliu C,Camins A,Pallàs M,del Valle J||Age (Dordrecht, Netherlands) (35:1851)||2013|
The protein phosphatase PP2A/Bα binds to the microtubule-associated proteins Tau and MAP2 at a motif also recognized by the kinase Fyn: implications for tauopathies.
||Sontag JM,Nunbhakdi-Craig V,White CL,Halpain S,Sontag E||The Journal of biological chemistry (287:14984)||2012|
Differing effects of toxicants (methylmercury, inorganic mercury, lead, amyloid β, and rotenone) on cultured rat cerebrocortical neurons: differential expression of rho proteins associated with neurotoxicity.
||Fujimura M,Usuki F||Toxicological sciences : an official journal of the Society of Toxicology (126:506)||2012|
Inhibition of cyclin-dependent kinase 5 but not of glycogen synthase kinase 3-β prevents neurite retraction and tau hyperphosphorylation caused by secretable products of human T-cell leukemia virus type I-infected lymphocytes.
AHB0042 was used in western blot to test if neurite retraction in the SH-SY5Y model is associated with changes in other tau hyperphosphorylable residues.
|Maldonado H,Ramírez E,Utreras E,Pando ME,Kettlun AM,Chiong M,Kulkarni AB,Collados L,Puente J,Cartier L,Valenzuela MA||Journal of neuroscience research (89:1489)||2011|
Deletion of tau attenuates heat shock-induced injury in cultured cortical neurons.
||Miao Y,Chen J,Zhang Q,Sun A||Journal of neuroscience research (88:102)||2010|
Defining Cdk5 ligand chemical space with small molecule inhibitors of tau phosphorylation.
||Ahn JS,Radhakrishnan ML,Mapelli M,Choi S,Tidor B,Cuny GD,Musacchio A,Yeh LA,Kosik KS||Chemistry & biology (12:811)||2005|
Neurite extension in central neurons: a novel role for the receptor tyrosine kinases Ror1 and Ror2.
||Paganoni S,Ferreira A||Journal of cell science (118:433)||2005|
Tau phosphorylation by cyclin-dependent kinase 5/p39 during brain development reduces its affinity for microtubules.
||Takahashi S,Saito T,Hisanaga S,Pant HC,Kulkarni AB||The Journal of biological chemistry (278:10506)||2003|
Tau reduction prevents disease in a mouse model of Dravet syndrome.
AHB0042 was used in immunohistochemistry to assess if tau reduction benefits intractable genetic epilepsies
|Gheyara AL,Ponnusamy R,Djukic B,Craft RJ,Ho K,Guo W,Finucane MM,Sanchez PE,Mucke L||Annals of neurology (76:443)||2014|
Ubiquitin-positive neuronal and tau 2-positive glial inclusions in frontotemporal dementia of motor neuron type.
||Forno LS,Langston JW,Herrick MK,Wilson JD,Murayama S||Acta neuropathologica (103:599)||2002|
Modulation of microtubule dynamics by tau in living cells: implications for development and neurodegeneration.
||Bunker JM,Wilson L,Jordan MA,Feinstein SC||Molecular biology of the cell (15:2720)||2004|
Development of an assay to screen for inhibitors of tau phosphorylation by cdk5.
||Ahn JS,Musacchio A,Mapelli M,Ni J,Scinto L,Stein R,Kosik KS,Yeh LA||Journal of biomolecular screening (9:122)||2004|
MTBT2, BOS_19251, MTBT1, TAU, DDPAC, AW045860, RNPTAU, AI413597, Tau, PPND, Mtapt, tau, FTDP-17, pTau, MAPTL, MSTD
G protein beta1/gamma2 subunit-interacting factor 1, PHF-tau, microtubule-associated protein tau, microtubule-associated protein tau, isoform 4, neurofibrillary tangle protein, paired helical filament-tau, Tau microtubule-associated protein, microtubule associated protein tau, MAPT, Microtubule-associated protein tau, MTBT1