|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Western Blot (WB)||1:500|
|Western Blot (WB)||See 2 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Pig|
|Published species reactivity||Hamster Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified extracellular domain of recombinant human thyrotropin receptor.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-217 detects both glycosylated and nonglycosylated thyroid stimulating hormone receptor (TSHR) from porcine tissues as well as recombinant human TSHR.
MA3-217 has been successfully used in Western blot, immunocytochemistry, and ELISA procedures. By Western blot, this antibody detects a 50 kDa and a 63 kDa protein representing unglycosylated and glycosylated TSRH respectively, in transfected Sf9 cell extract. By ELISA MA3-217 has been used in TSH competitive inhibition studies and to detect the presence of human TSHR.
The MA3-217 antigen is purified extracellular domain of recombinant human thyrotropin receptor. This antibody recognizes an epitope between amino acid residues 32-41 of human TSHR.
The thyroid stimulating hormone (thyrotropin) receptor (TSHR) is expressed on the surface of thyrocyte cell membranes and is crucial for thyroid gland growth and function. TSHR and quote;s are targets for the pathogenesis of many autoimmune diseases including Graves and quote; disease and primary myxedema. This 7-transmembrane-domain protein contains 5 potential extracellular N-linked glycosylation sites. Antibodies to TSHR can affect receptor function in a number of different ways. TSHR antibodies which can inhibit TSH binding to the receptor are called TSH binding inhibitor immunoglobulins or TBIIs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: seven transmembrane helix receptor; thyroid stimulating hormone receptor, isoform 2; Thyroid-stimulating hormone receptor; Thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH-R; TSHR
Gene Aliases: CHNG1; hTSHR-I; LGR3; TSHR
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