|Tested species reactivity||Human, Pig|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified extracellular domain of recombinant human thyrotropin receptor.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-217 detects both glycosylated and nonglycosylated thyroid stimulating hormone receptor (TSHR) from porcine tissues as well as recombinant human TSHR.
MA3-217 has been successfully used in Western blot, immunocytochemistry, and ELISA procedures. By Western blot, this antibody detects a 50 kDa and a 63 kDa protein representing unglycosylated and glycosylated TSRH respectively, in transfected Sf9 cell extract. By ELISA MA3-217 has been used in TSH competitive inhibition studies and to detect the presence of human TSHR.
The MA3-217 antigen is purified extracellular domain of recombinant human thyrotropin receptor. This antibody recognizes an epitope between amino acid residues 32-41 of human TSHR.
The thyroid stimulating hormone (thyrotropin) receptor (TSHR) is expressed on the surface of thyrocyte cell membranes and is crucial for thyroid gland growth and function. TSHR and quote;s are targets for the pathogenesis of many autoimmune diseases including Graves and quote; disease and primary myxedema. This 7-transmembrane-domain protein contains 5 potential extracellular N-linked glycosylation sites. Antibodies to TSHR can affect receptor function in a number of different ways. TSHR antibodies which can inhibit TSH binding to the receptor are called TSH binding inhibitor immunoglobulins or TBIIs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Overexpression of thyroid hormone receptor beta1 is associated with thyrotropin receptor gene expression and proliferation in a human thyroid carcinoma cell line.
MA3-217 was used in western blot to investigate the association of thyroid hormone receptor beta1 and thyrotropin receptor
|Chen ST,Shieh HY,Lin JD,Chang KS,Lin KH||The Journal of endocrinology (165:379)||2000|
Differentiation of human orbital preadipocyte fibroblasts induces expression of functional thyrotropin receptor.
MA3-217 was used in immunocytochemistry to show that the differentiation of human orbital preadipocyte fibroblasts can increase their TSHr expression
|Valyasevi RW,Erickson DZ,Harteneck DA,Dutton CM,Heufelder AE,Jyonouchi SC,Bahn RS||The Journal of clinical endocrinology and metabolism (84:2557)||1999|