Western blot of Transglutaminase 4 in HeLa lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a Transglutaminase 4 polyclonal antibody (Product# PA5-37895) at a dilution of 1ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-NQPLQSYHQLKLEFS, from the internal region near N-terminus of TGM4 (aa49-63)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.