|Tested species reactivity||Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified porcine brain tubulin.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1/50 - 1/500|
|Western Blot (WB)||1/250 - 1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Tubulin protein is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Anti-microtubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.