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Shows rat mixed neuron/glial cultures stained with Rabbit anti-UCHL1 antibody (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.
|Tested species reactivity||Bovine, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human UCHL1 purified from E. coli.|
|Storage buffer||whole serum|
|Contains||10mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for UCHL1 (transient overexpression lysate), SH-SY5Y neuroblastoma lysate and rat spinal cord and peripheral nerve homogenate.
Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. The protein is extremely abundant representing 1-2% of total
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP95; protein gene product 9.5; ubiquitin C-terminal hydrolase; ubiquitin C-terminal hydrolase-L1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCH-L1
AW822034; BOS_6576; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; UCH-L1; UCHL-1; UCHL1