Invitrogen

V-ATPase G3 Polyclonal Antibody

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V-ATPase G3 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of V-ATPase G3 in paraffin-embedded mouse kidney using V-ATPase G3 Polyclonal Antibody (Product # PA5-89832) at a dilution of 1:100.

V-ATPase G3 Antibody in Western Blot (WB) — Western Blot analysis of V-ATPase G3 in extracts of mouse kidney using V-ATPase G3 Polyclonal Antibody (Product # PA5-89832) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-89832

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human ATP6V1G3 View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2805796

Product Specific Information

Immunogen sequence: MTSQSQGIHQ LLQAEKRAKD KLEEAKKRKG KRLKQAKEEA MVEIDQYRMQ RDKEFRLKQS KIMGSQNNLS DEIEEQTLGK IQELNGHYNK YMESVMNQLL SMVCDMKPEI HVNYRATN; Positive Samples: Mouse kidney

Target Information

ATP6V1G3 (V-ATPase G3) is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3; ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3; ATPase, H+ transporting, V1 subunit G, isoform 3; V-ATPase 13 kDa subunit 3; V-ATPase G subunit 3; V-ATPase G3 subunit; V-ATPase subunit G 3; V-type proton ATPase subunit G 3; vacuolar ATP synthase subunit G 3; vacuolar proton pump G subunit 3; Vacuolar proton pump subunit G 3; vacuolar proton pump, subunit G3

Gene Aliases: ATP6G3; ATP6V1G3; Vma10

UniProt ID: (Human) Q96LB4, (Mouse) Q8BMC1

Entrez Gene ID: (Human) 127124, (Mouse) 338375

Molecular Function: ATP synthase