Invitrogen

VAMP8 Recombinant Rabbit Monoclonal Antibody (JF0963)

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Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

VAMP8 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of VAMP8 in SW480 cells using a VAMP8 Monoclonal antibody (Product # MA5-32502) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

VAMP8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of VAMP8 of paraffin-embedded Human kidney tissue using a VAMP8 Monoclonal antibody (Product #MA5-32502). Counter stained with hematoxylin.

VAMP8 Antibody in Western Blot (WB) — Western blot analysis of VAMP8 in different lysates using a Monoclonal antibody (Product #MA5-32502) at a dilution of 1:1,000. Positive control: Lane 1: Hela, Lane 2: Mouse kidney.

VAMP8 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of VAMP8 in Hela cells using a VAMP8 Monoclonal Antibody (Product # MA5-32502) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32502

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:100

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JF0963

Immunogen

Synthetic peptide wiithin Human VAMP8 aa 1-29

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809779

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EDB; Endobrevin; vamp; VAMP-8; Vesicle-associated membrane protein 8; vesicle-associated membrane protein 8 (endobrevin)

Gene Aliases: AU041171; EDB; endobrevin; VAMP-8; VAMP8

UniProt ID: (Human) Q6IB09, (Mouse) O70404, (Rat) Q9WUF4

Entrez Gene ID: (Human) 8673, (Mouse) 22320, (Rat) 83730

Function(s)

protein complex assembly transport exocytosis vesicle fusion autophagy protein transport vesicle-mediated transport regulation of endocytosis SNARE complex assembly viral entry into host cell membrane fusion mucus secretion autophagosome maturation regulation of protein localization to plasma membrane negative regulation of secretion by cell positive regulation of histamine secretion by mast cell endocytosis eosinophil degranulation neutrophil degranulation post-Golgi vesicle-mediated transport

Process(es)

SNARE binding SNAP receptor activity protein binding chloride channel inhibitor activity syntaxin binding