Invitrogen

VCAM-1 Monoclonal Antibody (1.4C3)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

8 References

View all (73) VCAM-1 (CD106) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

VCAM-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm of HUVEC cells treated with human TNF-alpha (right) compared to untreated HUVEC cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 monoclonal antibody (Product # MA5-11447) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

VCAM-1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of VCAM1 in 4% PFA, OCT embedded frozen mouse E16.5 lung sections. Sections were blocked and probed with a VCAM1 mouse monoclonal antibody (Product # MA5-11447) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-mouse secondary antibody (Product # A-11004).

Modulation of target protein phosphorylation by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of VCAM-1 using VCAM-1 Antibody (Product # MA5-11447) shows induced expression of VCAM-1 in the HUVEC cells upon TNF-alpha treatment. {TM}

Product Details

MA5-11447

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:12.5-1:25	-
Immunohistochemistry (Frozen) (IHC (F))	1:12.5-1:25	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1.4C3
Immunogen	Stimulated human umbilical vein endothelial cells (HUVEC)
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10979792

Product Specific Information

MA5-11447 targets CD106 in IHC (P, F) and ICC/IF applications and shows reactivity with Human samples.

The MA5-11447 immunogen is stimulated human umbilical vein endothelial cells (HUVEC).

MA5-11447 has been successfully used in immunohistochemistry analysis of VCAM1 in mouse E16.5 frozen lung sections.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Acute Graft-vs.-Host Disease-Associated Endothelial Activation in vitro Is Prevented by Defibrotide.

Frontiers in immunology

Martinez-Sanchez J,Hamelmann H,Palomo M,Mir E,Moreno-Castaño AB,Torramade S,Rovira M,Escolar G,Cordes S,Kalupa M,Mertlitz S,Riesner K,Carreras E,Penack O,Diaz-Ricart M

MA5-11447 was used in Immunocytochemistry-immunoflourescence to show that defibrotide has notable positive effects on endothelial biology during acute graft-vs.-host disease.

Wed Oct 28 00:00:00 EDT 2020

Glucocorticoid regulates mesenchymal cell differentiation required for perinatal lung morphogenesis and function.

American journal of physiology. Lung cellular and molecular physiology

Bridges JP,Sudha P,Lipps D,Wagner A,Guo M,Du Y,Brown K,Filuta A,Kitzmiller J,Stockman C,Chen X,Weirauch MT,Jobe AH,Whitsett JA,Xu Y

MA5-11447 was used in Immunohistochemistry to conclude that regulating signals controlling AT2/AT1 progenitor cell proliferation and differentiation and identifying cells and processes by which glucocorticoid signaling regulates fetal lung maturation.

Sat Aug 01 00:00:00 EDT 2020

Innate Immune Dysfunction in Rosacea Promotes Photosensitivity and Vascular Adhesion Molecule Expression.

The Journal of investigative dermatology

Kulkarni NN,Takahashi T,Sanford JA,Tong Y,Gombart AF,Hinds B,Cheng JY,Gallo RL

MA5-11447 was used in Immunohistochemistry to show how the expression of LL-37 can lead to enhanced sensitivity to UVB radiation in rosacea.

Sun Mar 01 00:00:00 EST 2020

Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression.

Theranostics

Li X,Chen W,Li P,Wei J,Cheng Y,Liu P,Yan Q,Xu X,Cui Y,Gu Z,Simoncini T,Fu X

Published figure using VCAM-1 monoclonal antibody (Product # MA5-11447) in Immunofluorescence

Mon Jul 09 00:00:00 EDT 2018

Inhibition of cell surface expression of endothelial adhesion molecules by ursolic acid prevents intimal hyperplasia of venous bypass grafts in rats.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Zeller I,Wiedemann D,Schwaiger S,Stelzmüller M,Kreutmayer S,Leberfing O,Stuppner H,Bernhard D

MA5-11447 was used in immunohistochemistry to study the role of reduced endothelial VCAM-1 expresson in the mechanism by which ursolic acid prevents intimal hyperplasia in a rat model of venous bypass graft

Thu Nov 01 00:00:00 EDT 2012

Identification and pharmacological characterization of the anti-inflammatory principal of the leaves of dwarf elder (Sambucus ebulus L.).

Journal of ethnopharmacology

Schwaiger S,Zeller I,Pölzelbauer P,Frotschnig S,Laufer G,Messner B,Pieri V,Stuppner H,Bernhard D

MA5-11447 was used in immunocytochemistry to identify and study the active anti-inflammatory component of dwarf elder leaves

Thu Jan 27 00:00:00 EST 2011

Leoligin, the major lignan from Edelweiss, inhibits intimal hyperplasia of venous bypass grafts.

Cardiovascular research

Reisinger U,Schwaiger S,Zeller I,Messner B,Stigler R,Wiedemann D,Mayr T,Seger C,Schachner T,Dirsch VM,Vollmar AM,Bonatti JO,Stuppner H,Laufer G,Bernhard D

MA5-11447 was used in flow cytometry to study the mechanism by which leoligin inhibits intimal hyperplasia of venous bypass grafts

Mon Jun 01 00:00:00 EDT 2009

Immunophenotypic profile and role of adhesion molecules in splenic marginal zone lymphoma with bone marrow involvement.

Leukemia & lymphoma

Florena AM,Tripodo C,Porcasi R,Ingrao S,Fadda MR,De Cantis S,Iannitto E,Franco V

MA5-11447 was used in immunohistochemistry to study the distribution and function of adhesion molecules in splenic marginal zone lymphoma

Sun Jan 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

Gene Aliases: CD106; INCAM-100; Vcam-1; VCAM1

UniProt ID: (Human) P19320

Entrez Gene ID: (Human) 7412, (Mouse) 22329

Function(s)