Invitrogen

VCAM-1 Monoclonal Antibody (1.4C3)

Advanced Verification

This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

Datasheet

Datasheet

Product Image Gallery

VCAM-1 Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human tonsil stained with CD106 antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of hematopoietic cells in germinal center.

VCAM-1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm of HUVEC cells treated with human TNF-alpha (right) compared to untreated HUVEC cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 monoclonal antibody (Product # MA5-11447) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

VCAM-1 Antibody — Published figure using VCAM-1 monoclonal antibody (Product # MA5-11447)

VCAM-1 Antibody in Cell Treatment — Modulation of target protein phosphorylation by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of VCAM-1 using VCAM-1 Antibody (Product # MA5-11447) shows induced expression of VCAM-1 in the HUVEC cells upon TNF-alpha treatment. {TM}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Frozen) (IHC (F))

1:12.5-1:25

Immunohistochemistry (Paraffin) (IHC (P))

1:12.5-1:25

Published Applications

Immunofluorescence (IF)

See 1 publication below

Immunohistochemistry (IHC)

See 2 publications below

Immunocytochemistry (ICC)

See 1 publication below

Flow Cytometry (Flow)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1.4C3

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10979792

Product Specific Information

MA5-11447 targets CD106 in IHC (P, F) and ICC/IF applications and shows reactivity with Human samples.

The MA5-11447 immunogen is stimulated human umbilical vein endothelial cells (HUVEC).

MA5-11447 has been successfully used in immunohistochemistry analysis of VCAM1 in mouse E16.5 frozen lung sections.

Target Information

CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110) is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. It is also present on follicular and interfollicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin alphaBeta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in tumor metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.