Invitrogen

VCAM-1 Monoclonal Antibody (1.4C3)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

5 References

View all (60) VCAM-1 (CD106) antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

VCAM-1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm of HUVEC cells treated with human TNF-alpha (right) compared to untreated HUVEC cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 monoclonal antibody (Product # MA5-11447) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

VCAM-1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of VCAM1 in 4% PFA, OCT embedded frozen mouse E16.5 lung sections. Sections were blocked and probed with a VCAM1 mouse monoclonal antibody (Product # MA5-11447) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-mouse secondary antibody (Product # A-11004).

VCAM-1 Antibody in Cell treatment — Modulation of target protein phosphorylation by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of VCAM-1 using VCAM-1 Antibody (Product # MA5-11447) shows induced expression of VCAM-1 in the HUVEC cells upon TNF-alpha treatment. {TM}

VCAM-1 Antibody — Published figure using VCAM-1 monoclonal antibody (Product # MA5-11447)

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:100

View 1 publication 1 publication

Immunofluorescence (IF)

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:12.5-1:25

Immunohistochemistry (Paraffin) (IHC (P))

1:12.5-1:25

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1.4C3

Immunogen

Stimulated human umbilical vein endothelial cells (HUVEC)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10979792

Product Specific Information

MA5-11447 targets CD106 in IHC (P, F) and ICC/IF applications and shows reactivity with Human samples.

The MA5-11447 immunogen is stimulated human umbilical vein endothelial cells (HUVEC).

MA5-11447 has been successfully used in immunohistochemistry analysis of VCAM1 in mouse E16.5 frozen lung sections.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.