Invitrogen

VCAM-1 Monoclonal Antibody (1.G11B1)

Product Image Gallery

VCAM-1 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of VCAM-1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VCAM-1 Mouse monoclonal Antibody (Product # MA5-16429) at 5 µg/ml in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2500 for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

VCAM-1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of KM-H2 cells stained with Mouse anti Human CD106

VCAM-1 Antibody — Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429)

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay Dependent

Flow Cytometry (Flow)

1:10 -1:50

View 3 publications 3 publications

Functional Assay (FN)

Assay Dependent

Immunocytochemistry (ICC)

5 µg/ml

Immunofluorescence (IF)

5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

Assay Dependent

Immunoprecipitation (IP)

Assay Dependent

Western Blot (WB)

Assay Dependent

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Neutralization (Neu)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human, Non-human primate, Pig

Published species

Human

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1.G11B1

Immunogen

human endothelial cells

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

4°C or -20°C if preferred

RRID

AB_2537945

Product Specific Information

Non-reducing conditions are recommended for Western blot applications.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

Gene Aliases: CD106; INCAM-100; VCAM1

UniProt ID: (Human) P19320

Entrez Gene ID: (Human) 7412, (Pig) 396925

Molecular Function: G-protein coupled receptor cell adhesion molecule cytokine receptor defense/immunity protein hydrolase immunoglobulin receptor superfamily immunoglobulin superfamily cell adhesion molecule phosphatase protein phosphatase receptor