Invitrogen

VCAM-1 Monoclonal Antibody (1.G11B1)

Product Image Gallery

VCAM-1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of VCAM-1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VCAM-1 Mouse monoclonal Antibody (Product # MA5-16429) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2500 for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

VCAM-1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of KM-H2 cells stained with Mouse anti Human CD106

VCAM-1 Antibody in Western Blot, Neutralization, Inhibition Assays (WB, Neu, IA) — Figure 2 The enhanced expression of ICAM-1 or VCAM-1 is not responsible for the PT-triggered tumor cell adhesion (A/E) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 12 h. The mRNA expression of ICAM-1 (A) or VCAM-1 (E) was analyzed by qPCR experiments. (B/F) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The total protein expression of ICAM-1 (B) or VCAM-1 (F) was determined by western blot analysis. (C/G) Confluent HUVECs were treated with PT (30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The cell surface expression of ICAM-1 (C) or VCAM-1 (G) was analyzed by flow cytometry. (D/H) Confluent HUVECs were treated with 30 nM PT for 24 h. An ICAM-1 blocking antibody (1, 5, 10 mug/ml) (D) or a VCAM-1 blocking antibody (1, 5, 10 mug/ml) (H) was added for the last 30 min of PT treatment. Fluorescence-labeled MDA cells were added and were allowed to adhere for 10 min. The amount of adherent MDA cells was determined by fluorescence measurements. (A-H) Data are expressed as mean +- SEM. A-C and E-H: n=3, D: n=5. *p <= 0.05 versus negative control, n.s.: p > 0.05 versus PT alone. TNFalpha was used as positive control and was not included into the statistical analyses.

Product Details

MA5-16429

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	5 µg/ml	-
Flow Cytometry (Flow)	1:10 -1:50	View 3 publications 3 publications
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	-
Neutralization (Neu)	-	View 3 publications 3 publications
Functional Assay (FN)	Assay-dependent	-
Inhibition Assays (IA)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Non-human primate, Pig
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	1.G11B1
Immunogen	human endothelial cells View antigen
Conjugate	Unconjugated FITC
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2537945

Product Specific Information

Non-reducing conditions are recommended for Western blot applications.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 EDT 2017

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 EDT 2017

The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.

Oncotarget

Schwenk R,Stehning T,Bischoff I,Ullrich A,Kazmaier U,Fürst R

Published figure using VCAM-1 monoclonal antibody (Product # MA5-16429) in Inhibition Assays

Fri Sep 29 00:00:00 EDT 2017

Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.

Cells, tissues, organs

Wagner W,Wein F,Roderburg C,Saffrich R,Diehlmann A,Eckstein V,Ho AD

MA5-16429 was used in blocking or activating experiment to test whether CD44 aids in adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells

Fri Sep 19 00:00:00 EDT 2008

Effects of vascular endothelial growth factor on the lymphocyte-endothelium interactions: identification of caveolin-1 and nitric oxide as control points of endothelial cell anergy.

Journal of immunology (Baltimore, Md. : 1950)

Bouzin C,Brouet A,De Vriese J,Dewever J,Feron O

MA5-16429 was used in blocking or activating experiment to clarify mechanisms that regulate tumor endothelial cells anergy

Thu Feb 01 00:00:00 EST 2007

Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells.

Histochemistry and cell biology

Neumüller J,Neumüller-Guber SE,Lipovac M,Mosgoeller W,Vetterlein M,Pavelka M,Huber J

MA5-16429 was used in flow cytometry to assess the ability of endothelial progenitor cells to differentiate into early mature endothelial cells

Fri Dec 01 00:00:00 EST 2006

Effect of maternal anti-HPA-1a antibodies and polyclonal IVIG on the activation status of vascular endothelial cells.

Clinical and experimental immunology

Radder CM,Beekhuizen H,Kanhai HH,Brand A

MA5-16429 was used in flow cytometry to test if anti-HPA-1a antibodies and intravenous immunoglobulin affect vascular endothelial cells in order to identify susceptibility for intracranial hemorrhage

Thu Jul 01 00:00:00 EDT 2004

Irradiation of mechanically-injured human arterial endothelial cells leads to increased gene expression and secretion of inflammatory and growth promoting cytokines.

Atherosclerosis

Wondergem J,Wedekind LE,Bart CI,Chin A,van der Laarse A,Beekhuizen H

MA5-16429 was used in flow cytometry to assess the effect of irradiation on re-endothelialisation of circular denuded tracks made in post-confluent cultures of arterial endothelial cells and cellular factors that contribute to this process

Thu Jul 01 00:00:00 EDT 2004

Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes.

The Journal of heart valve disease

Dvorin EL,Jacobson J,Roth SJ,Bischoff J

MA5-16429 was used in flow cytometry to explore how the cardiac valve endothelium is not damaged after transplantation.

Mon Sep 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

Gene Aliases: CD106; INCAM-100; VCAM1

UniProt ID: (Human) P19320

Entrez Gene ID: (Human) 7412, (Pig) 396925

Function(s)