Immunofluorescence analysis of von Willebrand Factor was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with von Willebrand Factor Mouse Monoclonal Antibody (MA514029) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing punctate cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Non-human primate, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Partially purified von Willebrand factor from human placenta|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14029 targets von Willebrand Factor in IHC (P) applications and shows reactivity with Human samples.
The MA5-14029 immunogen is partially purified von Willebrand factor from human placenta.
Factor VIII related antigen or von Willebrand factor is a multimeric glycoprotein, which binds to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. It is synthesized by endothelial cells and stored in the Weibel-Palade granules. It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms. As not all endothelial cells synthesize/ store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant. Staining for factor VIII related antigen has also been used to measure angiogenesis, an indicator of tumor recurrence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Microcirculatory effect of topical vapocoolants.
MA5-14029 was used in immunohistochemistry - frozen section to investigate the effects of vapocoolant spray administration on microcirculation or cutaneous blood flow
|Galdyn I,Swanson E,Gordon C,Kwiecien G,Bena J,Siemionow M,Zins J||Plastic surgery (Oakville, Ont.) (23:71)||2015|
Tracking fusion of human mesenchymal stem cells after transplantation to the heart.
MA5-14029 was used in immunohistochemistry to examine the migration of transplanted mesenchymal stem cells.
|Freeman BT,Kouris NA,Ogle BM||Stem cells translational medicine (4:685)||2015|
Lymphatic differentiation in classic Kaposi's sarcoma: patterns of D2-40 immunoexpression in the course of tumor progression.
MA5-14029 was used in immunohistochemistry to study the molecualr basis of lymphatic differentiation in classic Kaposi's sarcoma
|Kandemir NO,Barut F,Gun BD,Keser SH,Karadayi N,Gun M,Ozdamar SO||Pathology oncology research : POR (17:843)||2011|
Endothelium-coated tumor clusters are associated with poor prognosis and micrometastasis of hepatocellular carcinoma after resection.
MA5-14029 was used in immunohistochemistry to investigate the relationship between endothelium-coated tumor clusters and severity of liver cancer patients
|Ding T,Xu J,Zhang Y,Guo RP,Wu WC,Zhang SD,Qian CN,Zheng L||Cancer (117:4878)||2011|
Prognostic value of bone marrow microvessel density and angiogenic cytokines in patients with multiple myeloma undergoing autologous stem cell transplant.
MA5-14029 was used in immunohistochemistry to study the prognostic value of bone marrow microvessel density and angiogenic cytokines in patients undergoing autologous stem cell transplant
|Sucak GT,Aki SZ,Yüzba¿io¿lu B,Akyürek N,Ya¿ci M,Ba¿riaçik U,Haznedar R||Leukemia and lymphoma (52:1281)||2011|
Pulsed acoustic cellular treatment induces expression of proangiogenic factors and chemokines in muscle flaps.
MA5-14029 was used in immunohistochemistry to investigate the effect of pulsed acoustic cellular treatment on gene expression in muscle flaps
|Krokowicz L,Cwykiel J,Klimczak A,Mielniczuk M,Siemionow M||The Journal of trauma (69:1448)||2010|
The urokinase receptor supports tumorigenesis of human malignant pleural mesothelioma cells.
MA5-14029 was used in immunohistochemistry to investigate the role of urokinase receptor in the pathogenesis of malignant pleural mesothelioma
|Tucker TA,Dean C,Komissarov AA,Koenig K,Mazar AP,Pendurthi U,Allen T,Idell S||American journal of respiratory cell and molecular biology (42:685)||2010|
|Non-human primate||Not Cited||
Existence of the lymphatic system in the primate corpus luteum.
MA5-14029 was used in immunohistochemistry to detect the presence of lymphatic vessels in the corpus luteum
|Xu F,Stouffer RL||Lymphatic research and biology (7:159)||2009|
Epithelioid hemangioendothelioma derived from the spine region: case report and review of the literature.
MA5-14029 was used in immunohistochemistry to report on a case of epithelioid hemangioendothelioma
|Gokhan GA,Akyuz M,Gurer IE,Tuncer R||Wiener klinische Wochenschrift (118:358)||2006|
Heparanase is involved in angiogenesis in esophageal cancer through induction of cyclooxygenase-2.
MA5-14029 was used in immunohistochemistry to study the role of heparinase and cyclooxygenase-2 in angiogenesis in esophageal cancer
|Okawa T,Naomoto Y,Nobuhisa T,Takaoka M,Motoki T,Shirakawa Y,Yamatsuji T,Inoue H,Ouchida M,Gunduz M,Nakajima M,Tanaka N||Clinical cancer research : an official journal of the American Association for Cancer Research (11:7995)||2005|
Fibronectin promotes the phorbol 12-myristate 13-acetate-induced macrophage differentiation in myeloid leukemia cells.
MA5-14029 was used in immunocytochemistry to investigate the effect of fibronectin on macrophage differentiation of HL60 cells induced by phorbol 12-myristate 13-acetate
|Esendagli G,Canpinar H,Yilmaz G,Kaymaz FF,Kansu E,Guc D||International journal of hematology (89:167)||2009|
Isolation and characterization of human gingival microvascular endothelial cells.
MA5-14029 was used in immunocytochemistry to develop a method for isolation and characterization of human gingival microvascular endothelial cells
|DeCarlo AA,Cohen JA,Aguado A,Glenn B||Journal of periodontal research (43:246)||2008|
Regulation of Notch1 and Dll4 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis.
MA5-14029 was used in western blot to study the role of VEGF in regulating Notch1 and Dll4 expression in arterial endothelial cells
|Liu ZJ,Shirakawa T,Li Y,Soma A,Oka M,Dotto GP,Fairman RM,Velazquez OC,Herlyn M||Molecular and cellular biology (23:14)||2003|