Invitrogen

XRN1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

XRN1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of XRN1 was performed using SH-SY5Y and SH-SY5Y treated with BDNF (100 ng/mL, 72 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with XRN1 Polyclonal Antibody (Product # PA5-57110) at 5 µg/mL concentration in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d and e represents the merged image showing cytoplasmic localization. Expression of XRN1 was increased upon BDNF treatment in SH-SY5Y cells (Panel d) as compared to untreated SH-SY5Y cells (Panel e). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

XRN1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of XRN1 in human urinary bladder using a XRN1 Polyclonal Antibody (Product # PA5-57110) shows weak cytoplasmic positivity in urothelial cells.

XRN1 Antibody in Western Blot (WB) — Western blot was performed using anti-XRN1 Polyclonal Antibody (Product # PA5-57110) and a 180 kDa band corresponding to XRN1 was observed across all the untreated cell lines tested and was induced in SH-SY5Y upon BDNF treatment. Modified whole cell extracts (1%SDS) (30 µg lysate) of U-2 OS (Lane 1), SH-SY5Y (Lane 2), SH-SY5Y treated with BDNF (100 ng/mL for 72 hr) (Lane 3), NIH/3T3 (Lane 4), PC-3 (Lane 5) and LNCaP (Lane 6) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by wet transfer. The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

XRN1 Antibody in RNA Immunoprecipitation (RIP) — RNA Immunoprecipitation (RIP) assay of endogenous XRN1 protein using Anti-XRN1 Antibody: RIP assay was performed using Anti-XRN1 Recombinant Rabbit Polyclonal Antibody (Product # PA5-57110, 5 ug) on whole cell lysate from Hep G2 cells exposed to heat shock (45 degrees for 1 hour). Normal Rabbit IgG was used as a negative IP control. RNA purified by RiboPure™ RNA Purification Kit (Product # AM1924) was analyzed by RT-PCR using the Power SYBR® Green RNA-to-CT™ 1-Step Kit (Product # 4389986) with the primers pairs over IGF2, ACTB, MYC, RRP41, CCNA2, IGF2 mRNA and MALAT non-coding RNA. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

XRN1 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunocytochemistry using XRN1 Polyclonal Antibody (Product # PA5-57110), shows an increase in cytoplasmic levels of XRN1 in SH-SY5Y cells upon BDNF treatment. {TM}

Product Details

PA5-57110

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	-
Immunohistochemistry (IHC)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-
RNA Immunoprecipitation (RIP)	2.5 µg/10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human XRN1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2649702

Product Specific Information

Immunogen sequence: NVASSVLGKS VFVNWPHLEE ARVVAVSDGE TKFYLEEPPG TQKLYSGRTA PPSKVVHLGD KEQSNWAKEV QGISEHYLRR K

Highest antigen sequence identity to the following orthologs: Mouse - 84%, Rat - 85%.

Target Information

SEC24C is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The product of this gene may play a role in shaping the vesicle, as well as in cargo selection and concentration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 1-Sep; 5'-3' exoribonuclease 1; DKFZp434P0721; DKFZp686B22225; DKFZp686F19113; FLJ41903; protein Dhm2; Strand-exchange protein 1 homolog

Gene Aliases: Dhm2; exo; mXrn1; SEP1; XRN1

UniProt ID: (Human) Q68D88

Entrez Gene ID: (Human) 54464, (Mouse) 24127

Molecular Function: exoribonuclease