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ZFYVE1 Antibody (CF810112) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFYVE1 (RC211403, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFYVE. Positive lysates LY405962 (100 µg) and LC405962 (20 µg) can be purchased separately from OriGene.
Product Details
CF810112
Product Specifications
Species Reactivity
Rat,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI4H7
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-308 of human ZFYVE1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate (PtdIns(3)P)-containing membranes. This gene encodes a protein which contains two zinc-binding FYVE domains in tandem. This protein displays a predominantly Golgi, endoplasmic reticulum and vesicular distribution. Alternatively spliced transcript variants have been found for this gene, and they encode two isoforms with different sizes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Double FYVE-containing protein 1; phosphoinositide-binding protein SR3; protein phosphatase 1, regulatory subunit 172; SR3; Tandem FYVE fingers-1; tandem FYVE fingers-1 protein; Zinc finger FYVE domain-containing protein 1; zinc finger protein, subfamily 2A, member 1; zinc finger, FYVE domain containing 1
Gene Aliases: DFCP1; G630053K23; KIAA1589; mKIAA1589; PP10436; PPP1R172; SR3; TAFF1; ZFYVE1; ZNFN2A1
UniProt ID: (Human) Q9HBF4, (Mouse) Q810J8
Entrez Gene ID: (Human) 53349, (Mouse) 217695, (Rat) 299188
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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