Proteintech

beta Actin Monoclonal Antibody (2D4H5)

Product Image Gallery

beta Actin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 66009-1-IG (beta Actin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66009-1-Ig (beta actin Antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

beta Actin Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (beta Actin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

beta Actin Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug beta actin antibody (66009-1-IG, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IGG with dilution 1:100.

beta Actin Antibody in Immunoprecipitation (IP) — IP result of anti-beta actin (IP:66009-1-IG, 3ug; Detection:66009-1-IG 1:10000) with HeLa cells lysate 3100ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 335 publications 335 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 28 publications 28 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Rat, Zebrafish

Published species

Chicken, Fruit fly, Goat, Hamster, Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2D4H5

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody can recognize all the actins. The isotype of this antibody is IgG2b.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Atcb; B-ACTZF; beta cytoskeletal actin; Beta-actin; Beta-actin-1; Cap Z; capZ beta; cytoplasmic beta-actin; cytoskeleton actin; gamma actin; PS1TP5-binding protein 1

Gene Aliases: ACTB; actba; Actx; B-ACTZF; bact; bactin1; bactzf; Beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; wu:fd18f05

UniProt ID: (Human) P60709, (Chimpanzee) Q5R1X3, (Rat) P60711, (Mouse) P60710, (Chinese hamster) P48975, (Zebrafish) Q7ZVI7

Entrez Gene ID: (Human) 60, (Chimpanzee) 450133, (Rat) 81822, (Mouse) 11461, (Chinese hamster) 100689477, (Zebrafish) 57934

Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein