Search
GenScript
beta-tubulin Monoclonal Antibody (2G7D4)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
beta-tubulin Antibody (A01717-40) in WB
Western blot analysis of HeLa, HEK293, COS7, Pig muscle, Bovine muscle, Goat muscle, Rabbit muscle, Fish muscle, Mouse brain, and Rat brain lysates using β-tubulin Antibody (2G7D4), mAb, Mouse (GenScript, A01717, 1 µg/mL) The signal was developed with IRDye TM 800 Conjugated affinity Purified Goat Anti-Mouse IgG Predicted Size: 50 kD Observed Size: 50 kD.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A01717-40
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Fish,
Goat,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2G7D4
Immunogen
purified human recombinant full-length beta-tubulin protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Amount
40 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (a) and beta (β) chains. β-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots.GenScript β-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length β-tubulin protein.
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta 5-tubulin; beta Ib tubulin; beta tubulin; beta tubulin 1; beta-4 tubulin; beta-4-tubulin; beta-5 tubulin; beta-tubulin; beta-tubulin 1; beta-tubulin 2; beta-tubulin 3; beta-tubulin 4; beta-tubulin 5; beta-tubulin 6; beta-tubulin 7; beta-tubulin 8; beta-tubulin 9; Beta-tubulin class-I; beta-tubulin class-II; beta-tubulin class-III; beta-tubulin class-V; beta-tubulin class-VI; beta-tubulin T beta15 (aa 1-445); betaIII-tubulin; c(beta)7 tubulin; class II beta-tubulin isotype; class IIa beta-tubulin; class IIb beta-tubulin; class III beta-tubulin; class V beta-tubulin; dJ40E16.7; dystonia 4, torsion (autosomal dominant); epididymis secretory sperm binding protein; HSA10p15 beta-tubulin 4Q; M40 antibody; MGC8685; microtubule protein; neuron-specific class III beta-tubulin; similar to beta tubulin; T beta-15; TUB; TUB1; TUB2; TUB3; TUB4; TUB5; TUB6; TUB7; TUB8; TUB9; TUBB; tubulin beta; tubulin beta 4'; tubulin beta chain; tubulin beta class V; tubulin beta MGC4083; Tubulin beta-1 chain; tubulin beta-2A chain pseudogene; Tubulin beta-2B chain; tubulin beta-4 chain; tubulin beta-5 chain; tubulin beta-III; tubulin, beta 1; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIa; tubulin, beta 2B; tubulin, beta 3; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 6; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta polypeptide paralog; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 5; unnamed protein product; XTP3TPATP1
Gene Aliases: 2310057H16Rik; 2410129E14Rik; 2810484G07Rik; 3200002H15Rik; B130022C14Rik; bA506K6.1; bA631M21.2; beta-4; beta-5; brdp; CDCBM; CDCBM1; CDCBM5; CDCBM6; CDCBM7; CFEOM3; CFEOM3A; CSCSC1; DYT4; FEOM3; FPVEPD; HsT1601; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; MACTHC1; OK/SW-cl.56; OOMD; OOMD2; OZEMA2; PMGYSA; RGD1305887; RGD1309427; TUBB; TUBB-5; TUBB1; TUBB2; TUBB2A; Tubb2b; TUBB4; TUBB5
UniProt ID: (Chicken) P09244, (Human) P07437, (Pig) Q767L7, (Bovine) Q2KJD0, (Chicken) P09207, (Human) Q9H4B7, (Human) Q13885, (Chicken) P32882, (Human) Q9BVA1, (Bovine) Q6B856, (Human) Q13509, (Bovine) Q2T9S0, (Bovine) Q3ZBU7, (Human) P04350, (Human) Q9BUF5, (Chicken) P09653, (Bovine) Q2HJ81, (Human) Q3ZCM7, (Mouse) A2AQ07, (Mouse) Q7TMM9, (Rat) P85108, (Rat) Q3KRE8, (Mouse) Q9CWF2, (Mouse) Q9ERD7, (Rat) Q4QRB4, (Mouse) Q9D6F9, (Rat) P69897, (Mouse) P99024, (Mouse) Q922F4
Entrez Gene ID: (Rabbit) 100349792, (Goat) 102168953, (Chicken) 396254, (Human) 203068, (Pig) 733686, (Rabbit) 100358689, (Bovine) 615087, (Chicken) 396427, (Pig) 100134983, (Bovine) 541271, (Goat) 102188330, (Rabbit) 100347858, (Human) 81027, (Human) 7280, (Chicken) 768337, (Goat) 108633269, (Chicken) 420883, (Human) 347733, (Bovine) 281555, (Pig) 100153507, (Human) 10381, (Bovine) 768070, (Chicken) 431043, (Bovine) 540236, (Goat) 102178062, (Pig) 100736584, (Rabbit) 100348290, (Human) 10382, (Pig) 100623583, (Rabbit) 100350252, (Human) 84617, (Goat) 102178409, (Chicken) 421037, (Bovine) 534206, (Human) 347688, (Mouse) 545486, (Rat) 679312, (Mouse) 22151, (Rat) 498736, (Rat) 291081, (Mouse) 73710, (Mouse) 22152, (Rat) 246118, (Mouse) 22153, (Rat) 29213, (Rat) 29214, (Mouse) 22154, (Mouse) 67951, (Rat) 307351
GTPase activity
structural constituent of cytoskeleton
GTP binding
ubiquitin protein ligase binding
MHC class I protein binding
structural molecule activity
protein binding
protein domain specific binding
GTPase activating protein binding
macromolecular complex binding
calcium ion binding
protein heterodimerization activity
peptide binding
netrin receptor binding
double-stranded RNA binding
tubulin
microtubule cytoskeleton organization
mitotic cell cycle
microtubule-based process
regulation of synapse organization
spindle assembly
cytoskeleton organization
cytoskeleton-dependent intracellular transport
natural killer cell mediated cytotoxicity
cell division
odontoblast differentiation
platelet formation
thyroid gland development
microtubule polymerization
thyroid hormone transport
platelet aggregation
neuron migration
cerebral cortex development
negative regulation of microtubule polymerization
positive regulation of axon guidance
axon guidance
neuron differentiation
netrin-activated signaling pathway
dorsal root ganglion development
oocyte maturation
spindle assembly involved in female meiosis
biological_process
modulation of synaptic transmission
embryonic brain development
sperm motility
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support