|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to cytoplasmic domain of human CD117 protein.|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is human gastrointestinal stromal tumor.
This antibody recognizes a protein of 145kDa, which is identified as CD117/p145kit. This antibody does not interfere with the binding of SCF to c-kit. It precipitates both the unoccupied as well as the occupied form of c-kit. The binding of the stem cell factor (SCF) to the c-kit-encoded receptor tyrosine kinase (Type III) stimulates a variety of biochemical responses that culminate in cellular proliferation, migration, or survival. C-kit plays an important role in hematopoiesis, melanogenesis, and gametogenesis. CD117, also called KIT or C-kit receptor, is a cytokine receptor on the surface of hematopoietic stem cells and other cells. CD117 gene encodes the human homolog of the proto-oncogene c-kit. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, and chronic myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Immunity and defense Stress response Oncogenesis Developmental processes Gametogenesis Embryogenesis Cell structure and motility Cell motility Cell proliferation and differentiation