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Immunofluorescent analysis of c-Myc (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a c-Myc monoclonal antibody (Product # MA1-980) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Yeast, Rat, Mouse, Human, Not Applicable, Xenopus|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1:50-1:200|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:50 - 1:1000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-980 detects c-myc protein and c-myc tagged proteins.
MA1-980 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, flow cytometry, and immunoprecipitation procedures.
The MA1-980 immunogen corresponds to the synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.
The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation. In addition, deregulated expression of c-Myc induces apoptosis in different cell types, with c-myc requiring p53 for apoptosis in many cell types. This fact indicates heterogeneous mechanisms for c-myc-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MYC is expressed in the stromal and epithelial cells of primary breast carcinoma and paired nodal metastases.
MA1-980 was used in immunohistochemistry to investigate markers expressed by stromal cells in breast cancer patients.
|Mundim FG,Pasini FS,Brentani MM,Soares FA,Nonogaki S,Waitzberg AF||Molecular and clinical oncology (3:506)||2015|
Identification of two novel Shank3 transcripts in the developing mouse neocortex.
MA1-980 was used in immunohistochemistry to study the role in the developing murine neocortex of two novel transcripts of the Shank3 synaptic scaffold protein
|Waga C,Asano H,Sanagi T,Suzuki E,Nakamura Y,Tsuchiya A,Itoh M,Goto Y,Kohsaka S,Uchino S||Journal of neurochemistry (128:280)||2014|
Relationship between EBV infection and expression of cellular proteins c-Myc, Bcl-2, and Bax in gastric carcinomas.
MA1-980 was used in immunohistochemistry to study the relationship between EBV infection and expression of c-Myc, Bcl-2, and Bax in gastric carcinomas
|Lima MA,Ferreira MV,Barros MA,Pardini MI,Ferrasi AC,Mota RM,Rabenhorst SH||Diagnostic molecular pathology : the American journal of surgical pathology, part B (17:82)||2008|
|Not Applicable||Not Cited||
VHL negatively regulates SARS coronavirus replication by modulating nsp16 ubiquitination and stability.
MA1-980 was used in western blot to identify nsp16 binding partners using a yeast two-hybrid screen
|Yu X,Chen S,Hou P,Wang M,Chen Y,Guo D||Biochemical and biophysical research communications (459:270)||2015|
|Not Applicable||Not Cited||
Transcriptional gene silencing by Arabidopsis microrchidia homologues involves the formation of heteromers.
MA1-980 was used in western blot to study the role of heteromer formation in transcriptional gene silencing by the Arabidopsis homologues of mouse MORC genes
|Moissiard G,Bischof S,Husmann D,Pastor WA,Hale CJ,Yen L,Stroud H,Papikian A,Vashisht AA,Wohlschlegel JA,Jacobsen SE||Proceedings of the National Academy of Sciences of the United States of America (111:7474)||2014|
Regulation of the transcriptional activation of the androgen receptor by the UXT-binding protein VHL.
MA1-980 was used in immunoprecipitation and western blot to study the mechanism by which the UXT-binding protein VHL modulates androgen receptor transcriptional activation
|Chen S,Chen K,Zhang Q,Cheng H,Zhou R||The Biochemical journal (456:55)||2013|
Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex.
MA1-980 was used in western blot to study the roles of GPER and CDK6 in the mechanisms of fulvestrant resistance in ER-positive breast cancer cells
|Giessrigl B,Schmidt WM,Kalipciyan M,Jeitler M,Bilban M,Gollinger M,Krieger S,Jäger W,Mader RM,Krupitza G||British journal of cancer (109:2751)||2013|
The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity.
MA1-980 was used in western blot to study the mechanism by which Ubc9-mediated SUMOylation of ZHX1 modulates its stability and functional activity
|Chen S,Yu X,Lei Q,Ma L,Guo D||Journal of cellular biochemistry (114:2323)||2013|
SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.
MA1-980 was used in western blot to study the roles of SIRT1, ATM and HDAC1 in the DNA double-strand break response of neurons and the therapeutic significance for neurodegenerative disease
|Dobbin MM,Madabhushi R,Pan L,Chen Y,Kim D,Gao J,Ahanonu B,Pao PC,Qiu Y,Zhao Y,Tsai LH||Nature neuroscience (16:1008)||2013|
Functional impact of Aurora A-mediated phosphorylation of HP1γ at serine 83 during cell cycle progression.
MA1-980 was used in western blot to study the cell cycle role of HP1-gamma phosphorylation at Ser 83 by Aurora A
|Grzenda A,Leonard P,Seo S,Mathison AJ,Urrutia G,Calvo E,Iovanna J,Urrutia R,Lomberk G||Epigenetics & chromatin (6:null)||2013|
Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence.
MA1-980 was used in western blot to study the activation of the PRC-dependent stress program by apoptosis and senescence and its role in the response to cellular dysfunction
|Gleyzer N,Scarpulla RC||The Journal of biological chemistry (288:8004)||2013|
|Not Applicable||Not Cited||
The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.
MA1-980 was used in western blot to study the role of Aurora A kinase in the mechanism by which CUL3-KLHL18 regulates cell cycle entry
|Moghe S,Jiang F,Miura Y,Cerny RL,Tsai MY,Furukawa M||Biology open (1:82)||2012|
Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.
MA1-980 was used in western blot to study the interaction of Trf3 and Taf3 during early development and hematopoiesis in zebrafish
|Hart DO,Santra MK,Raha T,Green MR||Developmental dynamics : an official publication of the American Association of Anatomists (238:2540)||2009|
Akt phosphorylates both Tsc1 and Tsc2 in Drosophila, but neither phosphorylation is required for normal animal growth.
MA1-980 was used in western blot to investigate the phosphorylation of Tsc1 and Tsc2
|Schleich S,Teleman AA||PloS one (4:null)||2009|
Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo.
MA1-980 was used in western blot to study the results of adenoviral SERCA1 gene transfer in Sprague-Dawley rat hearts.
|O'Donnell JM,Fields A,Xu X,Chowdhury SA,Geenen DL,Bi J||American journal of physiology. Heart and circulatory physiology (295:H2483)||2008|
A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes.
MA1-980 was used in western blot to study the role of a Rictor-Myo1c complex in dynamic cortical actin events in 3T3-L1 adipocytes.
|Hagan GN,Lin Y,Magnuson MA,Avruch J,Czech MP||Molecular and cellular biology (28:4215)||2008|
DKC1 is a direct and conserved transcriptional target of c-MYC.
MA1-980 was used in western blot to investigate the relationship between DKC1 and c-MYC
|Alawi F,Lee MN||Biochemical and biophysical research communications (362:893)||2007|
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function.
MA1-980 was used in western blot to study the association between ubiquitin ligase cullin 7 binds and p53
|Andrews P,He YJ,Xiong Y||Oncogene (25:4534)||2006|
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
MA1-980 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes
|Wahl JK,Kim YJ,Cullen JM,Johnson KR,Wheelock MJ||The Journal of biological chemistry (278:17269)||2003|
14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin.
MA1-980 was used in western blot to study the role of 14-3-3beta in regulating the TSC2 tumor suppressor gene product tuberin
|Shumway SD,Li Y,Xiong Y||The Journal of biological chemistry (278:2089)||2003|
|Not Applicable||Not Cited||
Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells.
MA1-980 was used in immunocytochemistry to investigate the role of asymmetric cell division during tumorigenesis
|Izumi H,Kaneko Y||Cancer research (74:5620)||2014|
Functional analyses of melanocortin-4 receptor mutations identified from patients with binge eating disorder and nonobese or obese subjects.
MA1-980 was used in immunocytochemistry to study the role of MC4R mutations in the pathogenesis of binge eating disorder.
|Tao YX,Segaloff DL||The Journal of clinical endocrinology and metabolism (90:5632)||2005|
|Not Applicable||Not Cited||
Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway.
MA1-980 was used in immunocytochemistry to study the role of Myo1c in the membrane fusion of GLUT4-containing vesicles with the adipocyte plasma membrane
|Bose A,Robida S,Furcinitti PS,Chawla A,Fogarty K,Corvera S,Czech MP||Molecular and cellular biology (24:5447)||2004|
EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton.
MA1-980 was used in immunocytochemistry to study the role of EHD2 and EHBP1 in coupling endocytosis to the actin cytoskeleton
|Guilherme A,Soriano NA,Bose S,Holik J,Bose A,Pomerleau DP,Furcinitti P,Leszyk J,Corvera S,Czech MP||The Journal of biological chemistry (279:10593)||2004|
Deletion of codons 88-92 of the melanocortin-4 receptor gene: a novel deleterious mutation in an obese female.
MA1-980 was used in immunocytochemistry to investigate the role of the melanocortin-4 receptor in obesity.
|Donohoue PA,Tao YX,Collins M,Yeo GS,O'Rahilly S,Segaloff DL||The Journal of clinical endocrinology and metabolism (88:5841)||2003|
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
MA1-980 was used in immunocytochemistry to demonstrate that Ire1 plays a role in multiple facets of the ER stress-response
|Wang XZ,Harding HP,Zhang Y,Jolicoeur EM,Kuroda M,Ron D||The EMBO journal (17:5708)||1998|
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
MA1-980 was used in immunocytochemistry to study how Rat7p regulates nucleocytoplasmic export of mRNA
|Gorsch LC,Dockendorff TC,Cole CN||The Journal of cell biology (129:939)||1995|
MAPK Hog1 closes the S. cerevisiae glycerol channel Fps1 by phosphorylating and displacing its positive regulators.
MA1-980 was used in immunoprecipitation and western blot to study the regulation of the Saccharomyces cerevisiae Fps1 glycerol channel by Hog1-mediated phosphorylation
|Lee J,Reiter W,Dohnal I,Gregori C,Beese-Sims S,Kuchler K,Ammerer G,Levin DE||Genes & development (27:2590)||2013|
Detection of protein-RNA complexes in Xenopus oocytes.
MA1-980 was used in immunoprecipitation to develop a method for detecting protein-RNA complexes in Xenopus oocytes
|Huber PW,Zhao WM||Methods (San Diego, Calif.) (51:82)||2010|
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.
MA1-980 was used in immunoprecipitation to study the role of the WD40 protein FBW5 in promoting the ubiquitinylation of tumor suppresor protein TSC2
|Hu J,Zacharek S,He YJ,Lee H,Shumway S,Duronio RJ,Xiong Y||Genes & development (22:866)||2008|
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
MA1-980 was used in immunoprecipitation to investigate the interaction between ROC1 and cullin proteins
|Ohta T,Michel JJ,Schottelius AJ,Xiong Y||Molecular cell (3:535)||1999|
Array-based comparative genomic hybridization identifies CDK4 and FOXM1 alterations as independent predictors of survival in malignant peripheral nerve sheath tumor.
MA1-980 was used in immunohistochemistry - paraffin section to identify survival-associated genomic biomarkers for malignant peripheral nerve sheath tumors.
|Yu J,Deshmukh H,Payton JE,Dunham C,Scheithauer BW,Tihan T,Prayson RA,Guha A,Bridge JA,Ferner RE,Lindberg GM,Gutmann RJ,Emnett RJ,Salavaggione L,Gutmann DH,Nagarajan R,Watson MA,Perry A||Clinical cancer research : an official journal of the American Association for Cancer Research (17:1924)||2011|
avian myelocytomatosis viral oncogene homolog; c Myc; Cellular myelocytomatosis oncogene; class E basic helix-loop-helix protein 39; Myc proto oncogene protein; myc-related translation/localization regulatory factor; Myc2; Niard; Nird; proto-oncogene c-Myc; transcription factor p64; v myc avian myelocytomatosis viral oncogene hom; v-myc myelocytomatosis viral oncogene homolog
BHLHE39; c-Myc; MRTL; MYC; MYCC