Western blot analysis of delta Catenin in Cytosolic fraction of rat brain extract (Lanes A, C) and ECV304 whole cell extract (Lane B). Samples were probed with a delta Catenin polyclonal antibody (Product # PA1-25156). The antibody was developed with Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate. A, B: Antibody dilution at 1:2000. C: Antibody dilution at 1:2000 + 20 µg/ml p120ctn immunizing peptide.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 881-900 of human delta 1 Catenin (isoform 1B).|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25156 detects delta 1 Catenin from human samples.
PA1-25156 has been successfully used in Western blot procedures.
The PA1-25156 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 881-900 of human delta 1 Catenin (isoform 1B).
This gene encodes a member of the Armadillo protein family, which function in adhesion between cells and signal transduction. Multiple translation initiation codons and althernative splicing result in many different isoforms being translated. Not all of the full-length natures of the described transcript variants have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.