MA3-030 detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, human, mouse and rat tissues.
MA3-030 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and immunohistochemical experiments. By Western blot, this antibody detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS. Immunohistochemical staining of NOS with MA3-030 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.
The MA3-030 antigen is purified bovine bNOS.
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BNOS; cNOS; Constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; HEP-NOS; Hepatocyte NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, brain; Nitric oxide synthase, endothelial; Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nNOS; NOS type I; NOS type II; NOS type III; NOS, type II; NOSII; NOSIII; Peptidyl-cysteine S-nitrosylase NOS1; Peptidyl-cysteine S-nitrosylase NOS2
Gene Aliases: 2310005C01Rik; 2310065A03Rik; Bnos; ECNOS; eNOS; HEP-NOS; i-NOS; IHPS1; INOS; Inosl; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; Nos-2; Nos-3; NOS-I; NOS-II; NOS1; NOS2; NOS2A; NOS3