2D electrophoresis is used to separate proteins based upon their molecular weight after isoelectric focusing (IEF). 2D gels are generally designed to accept IEF tube gels, sample lanes sliced from vertical or horizontal IEF slab gels, or immobilized pH gradient (IPG) strips. Gels are usually run under denaturing conditions using SDS in the gel running buffers. ZOOM™ Gels are specifically designed for 2D separation of proteins that have been first separated by their pI on 7-cm IPG strips (e.g., ZOOM™ Strips). The IPG well of ZOOM™ Gels accommodates the full length of a 7-cm IPG strip (Figure 1). In addition to the IPG well, ZOOM™ Gels have a separate well designed for use with a molecular weight standard. The ZOOM™ cassette is the standard 10 cm x 10 cm format. ZOOM™ Gels are available in NuPAGE™ and Novex™ Tris-Glycine gel formulations.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)
XCell SureLock Mini-Cell, ZOOM IPGRunner Mini
Gel Percentage
4 to 20%
Gel Size
Mini
Gel Thickness
1.0 mm
Gel Type
Tris-Glycine
Product Type
Gradient Page
Sample Loading Volume
7 cm strips
Separation of
Protein
Separation Range
20 to 200 kDa
Separation Type
Denaturing
Shelf Life
8 Weeks
Wells
1-well
Mode of Separation
Molecular Weight
Product Line
Novex™, ZOOM™
Quantity
Each
Shipping Condition
Wet Ice
System Type
ZOOM™
Thickness
1.0 mm
Well Design
IPG Well
Length (Metric)
8 cm
Length (Metric) Gel
8 cm
Width (Metric)
8 cm
Width (Metric) Gel
8 cm
Contents & Storage
One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 8 weeks.