Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the
media selector tool.
This MEM is modified as follows:With | Without |
• Hanks' salts | • Sodium Bicarbonate |
• L-glutamine | |
• HEPES | |
The complete
formulation is available.
cGMP ManufacturingGibco™ MEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10%
Fetal Bovine Serum (FBS). This MEM formulation does not require a CO
2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see
protocol for details).