TrypLE™ Select Enzyme (1X), no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  12563029

Related applications:

Mammalian Cell Culture

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TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Select is:

Gentle on cells—maintains cell health for reproducible results
Room-temperature stable—convenient to use
Easy to use—substitutes directly into existing protocols, with no inactivation required
Animal origin free (AOF)—pure, consistent, and effective

This TrypLE™ Select is modified as follows:



• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

Product Intended Use
Customers using Gibco® TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.


Concentration: 1 X
Product Type: Cell Culture Dissociation Reagents
Chelators: EDTA
Classification: Animal Origin-Free
Endotoxin Level: Low
Enzyme Stability: Room Temperature Stable
pH Range: 7.0 - 7.4
Osmolality: 280 - 300 mOsm/kg
Shipping Condition: Room Temperature
Tested For: In Vitro Bioassay
Volume (Metric): 500 ml
Product Line: Gibco™
Form: Liquid
Phenol Red Indicator: No Phenol Red
Shelf Life: 24 Months
Green Features: Energy efficient, Sustainable packaging
Quantity: 500mL
Format: Bottle(s)
Reagent Type: Trypsin Replacement
Cell Type: Mammalian
Source: Animal origin-free
State at Room Temperature: 2°C to 8°C
pH: 7 to 7.4
Culture Type: Adherent Cell Culture

Contents & storage

Storage conditions: 15–30°C, protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.