Invitrogen™

Dynabeads™ Antibody Coupling Kit

Catalog number: 14311D
Have Questions?
Invitrogen™

Dynabeads™ Antibody Coupling Kit

Catalog number: 14311D
Have Questions?
Catalog Number
14311D
Unit Size
1 kit
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
14311D1 kit
-
Product Overview
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The Dynabeads™ Antibody Coupling Kit is intended for the coupling of an antibody or protein to the surface of Dynabeads™ M-270 Epoxy beads. The coupled beads and supplied buffers can then be used for experiments such as immunoassays, immunoprecipitation (IP), Co-IP of protein complexes, chromatin IP (ChIP), RNA-IP (RIP), cell isolation, etc. The Dynabeads Epoxy beads in this kit do not contain Tween™ detergent making them suitable for mass spectrometry (MS) analysis. The kit includes the buffers needed for efficient antibody coupling and washing steps. Note that the SB buffer supplied in the kit does contain Tween™ detergent, which means the buffer will need to be replaced with standard TBS or PBS buffer if the kit is to be used for MS.

Benefits of Dynabeads Antibody Coupling Kit include:
Convenience—contains optimized buffers and epoxy-activated Dynabeads magnetic beads
Efficiency­—easy-to-use protocol with minimal hands-on time
Flexibility­—allows coupling of any antibody of your choice
Robustness—covalent antibody coupling to the beads avoids co-elution of the antibody with the target proteins

Efficient and easy coupling
With this kit antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibodies contaminating the final eluate. Dynabeads M-270 Epoxy beads exhibit extremely low background binding and do not require blocking before use. Other proteins such as lectins and enzymes may also be coupled using the same beads, buffers, and protocol (depending on the stability and functionality of the protein/ligand you are using).

Magnetic bead-based separation offers easy handling
Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag magnet due to the magnetic properties of the Dynabeads M-270 Epoxy beads. The supplied buffers are optimized to give consistent and reliable results. In addition to Dynabeads M-270 Epoxy beads, the kit includes five different binding, washing, and elution buffers. Target specific antibody is required for coupling, but not included.

Automation-ready for use with KingFisher purification systems
The 2.8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems or liquid handlers.

Commercial supply
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Antibody Coupling Kit in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page.

Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:
Dynabeads M-270 Epoxy beads supplied in the kit can be purchased separately
See our immunoprecipitation selection table, data, and references
Dynabeads magnetic beads selection guide
See magnets for Dynabeads magnetic beads separations
KingFisher automation protocols
Watch a video about the KingFisher Flex instrument

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Certifications/Compliance
ISO9001 and ISO13485
Contents
Dynabeads M-270 Epoxy beads (60 mg) and 5 different buffers for covalent coupling and washing
Description
For immoblization of antibodies to magnetic beads
Color
Brown
For Use With (Application)
Immunoassays, Immunoprecipitation
Material
Polystyrene
Product Type
Antibody Coupling Kit
Final Product Type
Antibody (or other protein ligand) coupled Dynabeads
Format
Freeze-dried beads
For Use With (Equipment)
KingFisher™ Sample Purification Systems, DynaMag™ Magnets
High-throughput Compatibility
High-throughput Compatible
Isolation Technology
Magnetic beads
Sample Type
Intact Proteins (antibodies, purified antibodies, peptides, functional enzymes)
Shelf Life
24 months from date of manufacture
Shipping Condition
Ambient temperature
Surface Functionality
Epoxy
Uniformity
Monosized 2.8 μm (CV <5%)
Volume (Metric)
60 mg
Diameter (Metric)
2.8 μm
Regulatory Status
For Research Use Only

Contents & Storage

Store at 2°C to 8°C.

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