Invitrogen™

Uracil DNA Glycosylase

Catalog number: 18054015
Invitrogen™

Uracil DNA Glycosylase

Catalog number: 18054015
Catalog Number
18054015
also known as 18054-015
Unit Size
100 units
Price (USD)
183.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
18054015
also known as 18054-015
100 units
183.00
Your Price:
***
Product Overview
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Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.

Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).

Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).

Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.

Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.

Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 µg/ml 3H-poly(dU)n at 7 x 10 5 cpm/µg, and 1 unit of
enzyme in 50 µl for 10 min. at 37°C (4).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme
UDG
For Use With (Application)
PCR, Real Time PCR (qPCR)
Shipping Condition
Approved for shipment on Wet or Dry Ice
Form
Liquid
PCR Method
qPCR
Polymerase
DNA Polymerase

Contents & Storage

Store in freezer (-5 to -30°C).

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