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Citations & References (3)
Invitrogen™
Uracil DNA Glycosylase
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiesterRead more
| Catalog Number | Quantity |
|---|---|
| 18054015 | 100 units |
Catalog number 18054015
Price (MXN)
-
Quantity:
100 units
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 μg/ml 3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit of
enzyme in 50 μl for 10 min. at 37°C (4).
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 μg/ml 3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit of
enzyme in 50 μl for 10 min. at 37°C (4).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
PolymeraseDNA Polymerase
Quantity100 units
Shipping ConditionApproved for shipment on Wet or Dry Ice
For Use With (Application)Standard PCR, Real Time PCR (qPCR)
FormLiquid
PCR MethodqPCR
Unit SizeEach
Contents & Storage
Store in freezer (-5° to -30°C).
Frequently asked questions (FAQs)
What is UDG?
Citations & References (3)
Citations & References
Abstract
A novel MHC class I-like gene is mutated in patients with hereditary haemochromatosis.
Journal:Nat Genet
PubMed ID:10545944
Hereditary haemochromatosis (HH), which affects some 1 in 400 and has an estimated carrier frequency of 1 in 10 individuals of Northern European descent, results in multi-organ dysfunction caused by increased iron deposition, and is treatable if detected early. Using linkage-disequilibrium and full haplotype analysis, we have identified a 250-kilobase
Structural determinants for HIV-1 integrase inhibition by beta-diketo acids.
Journal:J Biol Chem
PubMed ID:11805103
Among all the HIV-1 integrase inhibitors, the beta-diketo acids (DKAs) represent a major lead in anti-HIV-1 integrase drug design. These derivatives inhibit the integration reaction in vitro with a strong specificity for the 3'-end joining step. They are also antiviral and inhibit integration in vivo. The aim of the present
Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates.
Journal:J Biol Chem
PubMed ID:11960995
The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG). MutY is a base excision repair (BER) glycosylase that removes misincorporated adenine residues from OG:A mispairs, as well as G:A and C:A mispairs. We