Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiesterRead more
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Catalog number 18054015
Price (USD)/ Each
232.00
-
Add to cart
Price (USD)/ Each
232.00
Add to cart
Uracil DNA Glycosylase
Catalog number18054015
Price (USD)/ Each
232.00
-
Add to cart
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 μg/ml 3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit of enzyme in 50 μl for 10 min. at 37°C (4).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormLiquid
PCR MethodqPCR
PolymeraseDNA Polymerase
Quantity100 units
Shipping ConditionApproved for shipment on Wet or Dry Ice
For Use With (Application)Standard PCR, Real Time PCR (qPCR)